Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HLA-C antibody [EPR6749] - BSA and Azide free (ab240027)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6749] to HLA-C - BSA and Azide free
  • Suitable for: IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-HLA-C antibody [EPR6749] - BSA and Azide free
    See all HLA-C primary antibodies

  • Description

    Rabbit monoclonal [EPR6749] to HLA-C - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Jurkat, HEK-293, A375, and HeLa whole cell lysates. Human lung and lymphoma tissue lysates; IP: Jurkat whole cell lysate.

  • General notes

    Ab240027 is the carrier-free version of ab126722. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240027 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-HLA-C antibody [EPR6749] - BSA and Azide free (ab240027)
    Western blot - Anti-HLA-C antibody [EPR6749] - BSA and Azide free (ab240027)
    All lanes : Anti-HLA-C antibody [EPR6749] (ab126722) at 1/5000 dilution (Purified)

    Lane 1 : Jurkat (Human Tcellleukemia Tlymphocyte) whole cell lysates
    Lane 2 : HEK-293 (Human embryonickidney epithelial cell) whole cell lysates
    Lane 3 : A375 (Human malignant melanoma epithelial cell) whole cell lysates
    Lane 4 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 5 : Human lung lysates
    Lane 6 : Human lymphoma lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 41 kDa
    Observed band size: 41 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126722).

  • Immunoprecipitation - Anti-HLA-C antibody [EPR6749] - BSA and Azide free (ab240027)
    Immunoprecipitation - Anti-HLA-C antibody [EPR6749] - BSA and Azide free (ab240027)

    Purified ab126722 at 1/100 dilution (2ug) immunoprecipitating HLA-C in Jurkat whole cell lysate.
    Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate (10µg)
    Lane 2 (+): ab126722 + Jurkat whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab126722 in Jurkat whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 41 kDa
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126722).

  • Anti-HLA-C antibody [EPR6749] - BSA and Azide free (ab240027)
    Anti-HLA-C antibody [EPR6749] - BSA and Azide free (ab240027)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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