Anti-HLA-C antibody [EPR6749] (ab126722)
![Anti-HLA-C antibody [EPR6749] (ab126722)](https://www.abcam.com/ps/products/126/ab126722/Images/ab126722-392501-anti-hla-c-antibody-epr6749-westernblot-human.jpg "Anti-HLA-C antibody [EPR6749] (ab126722)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6749] to HLA-C
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-HLA-C antibody [EPR6749]
See all HLA-C primary antibodies -
Description
Rabbit monoclonal [EPR6749] to HLA-C -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human HLA-C aa 350 to the C-terminus. The exact sequence is proprietary.
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Positive control
- WB: Jurkat, HEK-293, A375, and HeLa whole cell lysates. Human lung and lymphoma tissue lysates; IP: Jurkat whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6749 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-HLA-C antibody [EPR6749] (ab126722)All lanes : Anti-HLA-C antibody [EPR6749] (ab126722) at 1/5000 dilution (Purified)
Lane 1 : Jurkat (Human Tcellleukemia Tlymphocyte) whole cell lysates
Lane 2 : HEK-293 (Human embryonickidney epithelial cell) whole cell lysates
Lane 3 : A375 (Human malignant melanoma epithelial cell) whole cell lysates
Lane 4 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 5 : Human lung lysates
Lane 6 : Human lymphoma lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDaBlocking/Diluting buffer: 5% NFDM/TBST
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![Immunoprecipitation - Anti-HLA-C antibody [EPR6749] (ab126722)](https://www.abcam.com/ps/products/126/ab126722/Images/ab126722-3-anti-hla-c-antibody-epr6749-immunoprecipitation-jurkat-human.jpg)
Immunoprecipitation - Anti-HLA-C antibody [EPR6749] (ab126722)Purified ab126722 at 1/100 dilution (2ug) immunoprecipitating HLA-C in Jurkat whole cell lysate.
Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate (10µg)
Lane 2 (+): ab126722 + Jurkat whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab126722 in Jurkat whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 41 kDa -
![Anti-HLA-C antibody [EPR6749] (ab126722)](https://www.abcam.com/ps/products/126/ab126722/Images/ab126722-4-benefits-of-recombinant-antibodies.png)
Anti-HLA-C antibody [EPR6749] (ab126722)
