Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 02, 2021

Anti-GAD67 antibody [EPR20578] (ab213508)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20578] to GAD67
Suitable for: ICC, WB, IHC-P, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-GAD67 antibody [EPR20578]
See all GAD67 primary antibodies
Description

Rabbit monoclonal [EPR20578] to GAD67
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC	Mouse Rat
IHC-P	Mouse Rat
IP	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human cerebellum and fetal brain lysates; Mouse and rat cerebellum and brain lysates; IHC-P: Mouse and rat cerebral cortex, cerebellar cortex and pancreas tissues; IP: Human brain lysate; ICC: Mouse primary neuron and rat midbrain neuron cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20578
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD67 antibody [EPR20578] (ab213508)

Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling GAD67 with ab213508 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on mouse cerebral cortex (PMID: 25904362). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry - Anti-GAD67 antibody [EPR20578] (ab213508)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling GAD67 with ab213508 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing positive staining in mouse primary neuron cell. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab195889 Anti-alpha Tubulin antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).
Western blot - Anti-GAD67 antibody [EPR20578] (ab213508)

All lanes : Anti-GAD67 antibody [EPR20578] (ab213508) at 1/1000 dilution

Lane 1 : Mouse cerebellum lysate
Lane 2 : Mouse lung lysate
Lane 3 : Rat brain lysate
Lane 4 : Rat cerebellum lysate
Lane 5 : Rat lung lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 67 kDa
Observed band size: 67 kDa

Exposure time: 10 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

Negative control: Mouse lung, rat lung (PMID: 10671565).
Immunocytochemistry - Anti-GAD67 antibody [EPR20578] (ab213508)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat midbrain neuron cells labelling GAD67 with ab213508 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing positive staining in rat midbrain neuron cell. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (4 µg/mL) followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (2 µg/mL) (Red). The nuclear counterstain was DAPI (Blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD67 antibody [EPR20578] (ab213508)

Immunohistochemical analysis of paraffin-embedded mouse cerebellar cortex tissue labeling GAD67 with ab213508 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on mouse cerebellar cortex (PMID: 17235515). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD67 antibody [EPR20578] (ab213508)

Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling GAD67 with ab213508 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on islet of mouse pancreas (PMID: 8243826). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD67 antibody [EPR20578] (ab213508)

Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue labeling GAD67 with ab213508 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on rat cerebral cortex (PMID: 25904362). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD67 antibody [EPR20578] (ab213508)

Immunohistochemical analysis of paraffin-embedded rat cerebellar cortex tissue labeling GAD67 with ab213508 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on rat cerebellar cortex (PMID: 17235515). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD67 antibody [EPR20578] (ab213508)

Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling GAD67 with ab213508 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on islet of rat pancreas (PMID: 8243826). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-GAD67 antibody [EPR20578] (ab213508)

All lanes : Anti-GAD67 antibody [EPR20578] (ab213508) at 1/1000 dilution

Lane 1 : Human cerebellum lysate
Lane 2 : Human fetal brain lysate
Lane 3 : Human lung lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 67 kDa
Observed band size: 67 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

Negative control: Human lung (PMID: 10671565).
Western blot - Anti-GAD67 antibody [EPR20578] (ab213508)

Anti-GAD67 antibody [EPR20578] (ab213508) at 1/1000 dilution + Mouse brain lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 67 kDa
Observed band size: 67 kDa

Exposure time: 30 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunoprecipitation - Anti-GAD67 antibody [EPR20578] (ab213508)

GAD67 was immunoprecipitated from 0.35 mg of human brain lysate with ab213508 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab213508 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Lane 1: Human brain lysate 10 µg (Input).

Lane 2: ab213508 IP in human brain lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab213508 in human brain lysate.

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: 10 seconds.
Anti-GAD67 antibody [EPR20578] (ab213508)

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