Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GAD67 antibody [EPR20578] - BSA and Azide free (ab246335)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20578] to GAD67 - BSA and Azide free
  • Suitable for: WB, IP, IHC-P, ICC
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-GAD67 antibody [EPR20578] - BSA and Azide free
    See all GAD67 primary antibodies

  • Description

    Rabbit monoclonal [EPR20578] to GAD67 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-P, ICCmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse and rat cerebral cortex, cerebellar cortex and pancreas tissues; IP: Human brain lysate; ICC: Mouse primary neuron and rat midbrain neuron cells.

  • General notes

    Ab246335 is the carrier-free version of ab213508. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab246335 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD67 antibody [EPR20578] - BSA and Azide free (ab246335)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD67 antibody [EPR20578] - BSA and Azide free (ab246335)

    Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling GAD67 with ab213508 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on mouse cerebral cortex (PMID: 25904362). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213508).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry - Anti-GAD67 antibody [EPR20578] - BSA and Azide free (ab246335)
    Immunocytochemistry - Anti-GAD67 antibody [EPR20578] - BSA and Azide free (ab246335)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling GAD67 with ab213508 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing positive staining in mouse primary neuron cell. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab195889 Anti-alpha Tubulin antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213508).

  • Immunoprecipitation - Anti-GAD67 antibody [EPR20578] - BSA and Azide free (ab246335)
    Immunoprecipitation - Anti-GAD67 antibody [EPR20578] - BSA and Azide free (ab246335)

    GAD67 was immunoprecipitated from 0.35 mg of human brain lysate with ab213508 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab213508 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1:  Human brain lysate 10 µg (Input). 

    Lane 2: ab213508 IP in human brain lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab213508 in human brain lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213508).

  • Immunocytochemistry - Anti-GAD67 antibody [EPR20578] - BSA and Azide free (ab246335)
    Immunocytochemistry - Anti-GAD67 antibody [EPR20578] - BSA and Azide free (ab246335)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat midbrain neuron cells labelling GAD67 with ab213508 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing positive staining in rat midbrain neuron cell. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (4 µg/mL) followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (2 µg/mL) (Red). The nuclear counterstain was DAPI (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213508).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD67 antibody [EPR20578] - BSA and Azide free (ab246335)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD67 antibody [EPR20578] - BSA and Azide free (ab246335)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellar cortex tissue labeling GAD67 with ab213508 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on mouse cerebellar cortex (PMID: 17235515). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213508).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD67 antibody [EPR20578] - BSA and Azide free (ab246335)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD67 antibody [EPR20578] - BSA and Azide free (ab246335)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling GAD67 with ab213508 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on islet of mouse pancreas (PMID: 8243826). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213508).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD67 antibody [EPR20578] - BSA and Azide free (ab246335)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD67 antibody [EPR20578] - BSA and Azide free (ab246335)

    Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue labeling GAD67 with ab213508 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on rat cerebral cortex (PMID: 25904362). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213508).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD67 antibody [EPR20578] - BSA and Azide free (ab246335)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD67 antibody [EPR20578] - BSA and Azide free (ab246335)

    Immunohistochemical analysis of paraffin-embedded rat cerebellar cortex tissue labeling GAD67 with ab213508 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on rat cerebellar cortex (PMID: 17235515). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213508).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD67 antibody [EPR20578] - BSA and Azide free (ab246335)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD67 antibody [EPR20578] - BSA and Azide free (ab246335)

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling GAD67 with ab213508 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on islet of rat pancreas (PMID: 8243826). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213508).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-GAD67 antibody [EPR20578] - BSA and Azide free (ab246335)
    Anti-GAD67 antibody [EPR20578] - BSA and Azide free (ab246335)

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