Anti-OTP antibody [EPR22178-17] (ab254267)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22178-17] to OTP
- Suitable for: IHC-P
- Reacts with: Human
Overview
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Product name
Anti-OTP antibody [EPR22178-17]
See all OTP primary antibodies -
Description
Rabbit monoclonal [EPR22178-17] to OTP -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human lung carcinoid and lung small cell carcinoma tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22178-17 -
Isotype
IgG -
Research areas
Images
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Immunohistochemical analysis of paraffin-embedded human lung carcinoid tissue labeling OTP with ab254267 at 1/4000 dilution (0.113µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in human lung carcinoid (PMID: 30284410, 29316326) is observed. The expression of the target is developmental stage-specific and type-specific for lung cancer. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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Immunohistochemical analysis of paraffin-embedded human lung small cell carcinoma tissue labeling OTP with ab254267 at 1/4000 dilution (0.113µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in human lung small cell carcinoma (PMID: 26927888) is observed. The expression of the target is developmental stage-specific and type-specific for lung cancer. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue labeling OTP with ab254267 at 1/4000 dilution (0.113µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Negative control: No staining in human lung squamous cell carcinoma (PMID: 26927888) is observed. The expression of the target is developmental stage-specific and type-specific for lung cancer. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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Immunohistochemical analysis of paraffin-embedded human lung tissue labeling OTP with ab254267 at 1/4000 dilution (0.113µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Negative control: No staining in human lung (PMID: 26927888) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
-