Anti-FZR1 antibody [AR38.2] (ab77885)
![Anti-FZR1 antibody [AR38.2] (ab77885)](https://www.abcam.com/ps/products/77/ab77885/Images/ab77885-414935-anti-fzr1-antibody-ar38-2-western-blot-human-knockout-lysate.jpg "Anti-FZR1 antibody [AR38.2] (ab77885)")
Key features and details
- Mouse monoclonal [AR38.2] to FZR1
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-FZR1 antibody [AR38.2]
See all FZR1 primary antibodies -
Description
Mouse monoclonal [AR38.2] to FZR1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant full length protein
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Positive control
- WB: HEK293, HeLa and A431 cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
AR38.2 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-FZR1 antibody [AR38.2] (ab77885)All lanes : Anti-FZR1 antibody [AR38.2] (ab77885) at 1/500 dilution
Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : FZR1 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDaLanes 1-4: Merged signal (red and green). Green - ab77885 observed at 55 kDa. Red - loading control ab181602 observed at 36 kDa.
ab77885 Anti-FZR1 antibody [AR38.2] was shown to specifically react with FZR1 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab267263 (knockout cell lysate ab263208) was used. Wild-type and FZR1 knockout samples were subjected to SDS-PAGE. ab77885 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
