Anti-Apc7 antibody (ab4171)
Key features and details
- Rabbit polyclonal to Apc7
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Apc7 antibody -
Description
Rabbit polyclonal to Apc7 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human Apc7 aa 100-200 conjugated to keyhole limpet haemocyanin.
(Peptide available asab13734) -
Positive control
- This antibody gave a positive control in the following lysates: HeLa Nuclear Extract Heart (Mouse) Tissue Spinal Cord (Mouse) Tissue
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab4171 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB 1/500. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa). ICC/IF Use a concentration of 10 µg/ml. Target
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Function
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the APC7 family.
Contains 7 TPR repeats. - Information by UniProt
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Database links
- Entrez Gene: 51434 Human
- Entrez Gene: 56317 Mouse
- Omim: 606949 Human
- SwissProt: Q9UJX3 Human
- SwissProt: Q9WVM3 Mouse
- Unigene: 719935 Human
- Unigene: 37341 Mouse
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Alternative names
- anapc7 antibody
- Anaphase promoting complex subunit 7 antibody
- Anaphase-promoting complex subunit 7 antibody
see all
Images
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Western blot - Anti-Apc7 antibody (ab4171)All lanes : Anti-Apc7 antibody (ab4171)
Lane 1 : HeLa Nuclear Extract
Lane 2 : HeLa Nuclear Extract with Apc7 peptide (ab13734) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution
Lane 2 : Goat polyclonal to Rabbit IgG - H&L (HRP) (ab6721) at 1/2000 dilution
Predicted band size: 63 kDa
Observed band size: 63 kDa
Additional bands at: 39 kDa, 45 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minuteWestern blot using ab4171 against Hela Nuclear Extract.
The expected band size for Apc7 is 63 kd, which corresponds to the band seen in this western. The band is blocked by the immunising peptide.
Secondary ab: Goat anti-rabbit IgG HRP conjugate ab6721 (1/2000)
Exposure time: 1minLane 1 and 2: 20
µ g/lane HeLa NuclearLane 1: ab4171 (1/500)
Lane 2: ab4171 (1/500) + 1.0µ g ab4171 peptide -
Western blot - Anti-Apc7 antibody (ab4171)All lanes : Anti-Apc7 antibody (ab4171) at 1 µg/ml
Lane 1 : Heart (Mouse) Tissue Lysate
Lane 2 : Spinal Cord (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 63 kDa
Observed band size: 63 kDa
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands. -
Immunocytochemistry/ Immunofluorescence - Anti-Apc7 antibody (ab4171)ICC/IF image of ab4171 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4171, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
References (2)
ab4171 has been referenced in 2 publications.
- Izawa D & Pines J How APC/C-Cdc20 changes its substrate specificity in mitosis. Nat Cell Biol 13:223-33 (2011). WB ; Human . PubMed: 21336306
- Nilsson J et al. The APC/C maintains the spindle assembly checkpoint by targeting Cdc20 for destruction. Nat Cell Biol 10:1411-20 (2008). PubMed: 18997788
Images
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Western blot - Anti-Apc7 antibody (ab4171)All lanes : Anti-Apc7 antibody (ab4171)
Lane 1 : HeLa Nuclear Extract
Lane 2 : HeLa Nuclear Extract with Apc7 peptide (ab13734) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution
Lane 2 : Goat polyclonal to Rabbit IgG - H&L (HRP) (ab6721) at 1/2000 dilution
Predicted band size: 63 kDa
Observed band size: 63 kDa
Additional bands at: 39 kDa, 45 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minuteWestern blot using ab4171 against Hela Nuclear Extract.
The expected band size for Apc7 is 63 kd, which corresponds to the band seen in this western. The band is blocked by the immunising peptide.
Secondary ab: Goat anti-rabbit IgG HRP conjugate ab6721 (1/2000)
Exposure time: 1minLane 1 and 2: 20
µ g/lane HeLa NuclearLane 1: ab4171 (1/500)
Lane 2: ab4171 (1/500) + 1.0µ g ab4171 peptide -
Western blot - Anti-Apc7 antibody (ab4171)All lanes : Anti-Apc7 antibody (ab4171) at 1 µg/ml
Lane 1 : Heart (Mouse) Tissue Lysate
Lane 2 : Spinal Cord (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 63 kDa
Observed band size: 63 kDa
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.
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Immunocytochemistry/ Immunofluorescence - Anti-Apc7 antibody (ab4171)ICC/IF image of ab4171 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4171, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.