ANAPC2 overexpression 293T lysate (whole cell) (ab94319)
Overview
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Product name
ANAPC2 overexpression 293T lysate (whole cell) -
General notes
ab94319 is a 293T cell transfected lysate in which Human Apc2 has been transiently over-expressed using a pCMV-Apc2 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Research areas
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Background
Function: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Pathway: Protein modification; protein ubiquitination. Similarity: Belongs to the cullin family.
Images
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SDS-PAGE - ANAPC2 overexpression 293T lysate (whole cell) (ab94319)ab94319 at 15µg/lane on an SDS-PAGE gel.
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Western blot - ANAPC2 overexpression 293T lysate (whole cell) (ab94319)All lanes : Anti-Apc2 antibody (ab57094) at 1/500 dilution
Lane 1 :ANAPC2 overexpression 293T lysate (whole cell) (ab94319)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution