Anti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Chimeric (ab272852)
![Anti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Chimeric (ab272852)](https://www.abcam.com/ps/products/272/ab272852/Images/ab272852-389948-Mouse-monoclonal-1A6-to-SARSCoV2-nucleocapsid-protein-western-blot.jpg "Anti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Chimeric (ab272852)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Human monoclonal [1A6] to SARS-CoV-2 nucleocapsid protein - Chimeric
- Suitable for: WB, ELISA
Overview
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Product name
Anti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Chimeric
See all SARS-CoV-2 nucleocapsid protein primary antibodies -
Description
Human monoclonal [1A6] to SARS-CoV-2 nucleocapsid protein - Chimeric -
Host species
Human -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: SARS-CoV-2 -
Immunogen
Recombinant full length protein corresponding to SARS-CoV-2 nucleocapsid protein aa 1-419. Recombinant anti-SARS-CoV-2 Nucleoprotein Mouse ScFv is expressed from 293 cells (HEK293) with a human IgG1 Fc tag on C-terminal.
Database link: P0DTC9 -
Positive control
- ELISA: Human Novel Coronavirus (SARS-CoV-2/ 2020-nCoV) samples. WB: His tag-tagged SARS-CoV-2 nucleocapsid recombinant protein from E.coli.
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General notes
Anti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Chimeric (ab272852) is a recombinant human/mouse chimeric monoclonal antibody consisting of Mouse scFv fused with human IgG1.
As the Fc region is human, an anti-Human secondary antibody should be used for detection.
Applications overview
Tick: Tested and Guaranteed to work X: Will not work —: No data
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 0.03% Proclin 300, 50% Glycerol, PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Screened the positive clones from mouse ScFv antibody libraries, then expressed in 293F cells. -
Clonality
Monoclonal -
Clone number
1A6 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Chimeric (ab272852)Anti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Chimeric (ab272852) at 1/1000 dilution + His tag-tagged SARS-CoV-2 nucleocapsid recombinant protein from E.coli, 200 ng
Secondary
Peroxidase Goat Anti-Human IgG, Fc? Fragment Specific at 1/20000 dilution
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![ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Chimeric (ab272852)](https://www.abcam.com/ps/products/272/ab272852/Images/ab272852-390095-Anti-sars-cov2-nucleocapsid-protein-antibody-1a6-coronavirus-elisa.jpg)
ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Chimeric (ab272852)Binding Activity of ab272852 with SARS-CoV-2 nucleocapsid protein.Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2 nucleocapsid protein at 5 μg/ml can bind ab272852. The EC50 of ab272852 is 43.50-118.4 ng/ml. -
![Anti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Chimeric (ab272852)](https://www.abcam.com/ps/products/272/ab272852/Images/ab272852-5-benefits-of-recombinant-antibodies.png)
Anti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Chimeric (ab272852)