Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Chimeric (ab272852)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Human monoclonal [1A6] to SARS-CoV-2 nucleocapsid protein - Chimeric
  • Suitable for: WB, ELISA

Overview

  • Product name

    Anti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Chimeric
    See all SARS-CoV-2 nucleocapsid protein primary antibodies

  • Description

    Human monoclonal [1A6] to SARS-CoV-2 nucleocapsid protein - Chimeric

  • Host species

    Human

  • Tested applications

    Suitable for: WB, ELISAmore details

  • Species reactivity

    Reacts with: SARS-CoV-2

  • Immunogen

    Recombinant full length protein corresponding to SARS-CoV-2 nucleocapsid protein aa 1-419. Recombinant anti-SARS-CoV-2 Nucleoprotein Mouse ScFv is expressed from 293 cells (HEK293) with a human IgG1 Fc tag on C-terminal.
    Database link: P0DTC9

  • Positive control

    • ELISA: Human Novel Coronavirus (SARS-CoV-2/ 2020-nCoV) samples. WB: His tag-tagged SARS-CoV-2 nucleocapsid recombinant protein from E.coli.

  • General notes

    Anti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Chimeric (ab272852) is a recombinant human/mouse chimeric monoclonal antibody consisting of Mouse scFv fused with human IgG1.

    As the Fc region is human, an anti-Human secondary antibody should be used for detection.

    Applications overview

    Tick: Tested and Guaranteed to work      X: Will not work      —: No data

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Constituents: 0.03% Proclin 300, 50% Glycerol, PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    Screened the positive clones from mouse ScFv antibody libraries, then expressed in 293F cells.

  • Clonality

    Monoclonal

  • Clone number

    1A6

  • Isotype

    IgG1

  • Research areas

Images

  • Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Chimeric (ab272852)
    Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Chimeric (ab272852)
    Anti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Chimeric (ab272852) at 1/1000 dilution + His tag-tagged SARS-CoV-2 nucleocapsid recombinant protein from E.coli, 200 ng

    Secondary
    Peroxidase Goat Anti-Human IgG, Fc? Fragment Specific at 1/20000 dilution
  • ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Chimeric (ab272852)
    ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Chimeric (ab272852)
    Binding Activity of ab272852 with SARS-CoV-2 nucleocapsid protein.
    Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2 nucleocapsid protein at 5 μg/ml can bind ab272852. The EC50 of ab272852 is 43.50-118.4 ng/ml.
  • Anti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Chimeric (ab272852)
    Anti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Chimeric (ab272852)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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