Anti-BLBP antibody [EPR24033-13] (ab279649)
![Anti-BLBP antibody [EPR24033-13] (ab279649)](https://www.abcam.com/ps/products/279/ab279649/Images/ab279649-451603-anti-blbp-antibody-epr2403313-western-blot-lysate1.jpg "Anti-BLBP antibody [EPR24033-13] (ab279649)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24033-13] to BLBP
- Suitable for: WB, ICC, IHC-Fr, IHC-P, Flow Cyt (Intra), IP
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-BLBP antibody [EPR24033-13]
See all BLBP primary antibodies -
Description
Rabbit monoclonal [EPR24033-13] to BLBP -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt MouseRatICC MouseRatIHC-Fr MouseIHC-P MouseRatHumanIP MouseWB MouseRatHuman
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse brain and cerebellum tissue lysate. Human cerebellum tissue lysate. Rat brain cortex and cerebellum tissue lysate. IHC-P: Human, mouse and rat cerebrum tissue. Human astrocytoma. IHC-Fr: Mouse cerebellum tissue. ICC: Mouse and rat primary neural/glia cells. Flow Cyt: Mouse and rat primary neural/glia cells. IP: Mouse cerebellum tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24033-13 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-BLBP antibody (ab279649)All lanes : Anti-BLBP antibody [EPR24033-13] (ab279649) at 1/1000 dilution
Lane 1 : Human cerebellum tissue lysate
Lane 2 : Human spleen tissue lysate
Lane 3 : Human kidney tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: spleen, kidney (PMID: 16618771)
Exposure time: 10 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BLBP antibody (ab279649)Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling BLBP with ab279649 at 1/500 dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human cerebrum (PMID: 16623952). The section was incubated with ab279649 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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Immunohistochemistry (Frozen sections) - Anti-BLBP antibody (ab279649)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebellum tissue labeling BLBP with ab279649 at 1/50 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse cerebellum, which partially co-stained with GFAP (Red) is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1:1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).dilution. -
Immunocytochemistry - Anti-BLBP antibody (ab279649)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse primary neural/glia cells labelling BLBP with ab279649 at 1/250 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing positive staining in mouse primary gliocyte. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection is observed. Anti-Glial Fibrillary Acidic Protein (GFAP) mouse monoclonal antibody was used to counterstain tubulin at 1/100 dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at a 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).
Negative control 1: ab279649 at a 1/250 dilution followed by ab150120 at a 1/1000 dilution.
Negative control 2: Anti-Glial Fibrillary Acidic Protein (GFAP) mouse monoclonal antibody at a 1/100 dilution followed by ab150077 at a 1/1000 dilution.
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Flow Cytometry - Anti-BLBP antibody (ab279649)Flow cytometric analysis of 4% paraformaldehyde fixed 0.1% Tween-20 permeabilized mouse primary neural/glia cells labeling BLBP with ab279649 at 1/500 dilution (Right panel) compared with Rabbit monoclonal IgG Isotype Control (ab172730) (Left panel). Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody.
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Immunoprecipitation - Anti-BLBP antibody (ab279649)BLBP was immunoprecipitated from 0.35 mg mouse cerebellum tissue lysate 10 µg with ab279649 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab279649 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Mouse cerebellum tissue lysate 10 µg
Lane 2: ab279649 IP in mouse cerebellum tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab279649 in mouse cerebellum tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BLBP antibody (ab279649)Immunohistochemical analysis of paraffin-embedded human astrocytoma labeling BLBP with ab279649 at 1/500 dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human astrocytoma (PMID: 16623952). The section was incubated with ab279649 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BLBP antibody (ab279649)Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling BLBP with ab279649 at 1/500 dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab279649 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Negative control: no staining on human kidney (PMID: 9375786).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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Western blot - Anti-BLBP antibody (ab279649)All lanes : Anti-BLBP antibody [EPR24033-13] (ab279649) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse cerebellum tissue lysate
Lane 3 : Mouse spleen tissue lysate
Lane 4 : Mouse kidney tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: spleen, kidney (PMID: 16618771)
Exposure time: 6 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BLBP antibody (ab279649)Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling BLBP with ab279649 at 1/2000 dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse cerebrum. The section was incubated with ab279649 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BLBP antibody (ab279649)Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling BLBP with ab279649 at 1/2000 dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab279649 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Negative control: no staining on mouse kidney.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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Western blot - Anti-BLBP antibody (ab279649)All lanes : Anti-BLBP antibody [EPR24033-13] (ab279649) at 1/1000 dilution
Lane 1 : Rat brain cortex tissue lysate
Lane 2 : Rat cerebellum tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure times:
Lane 1: 3 seconds
Lane 2: 26 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BLBP antibody (ab279649)Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling BLBP with ab279649 at 1/2000 dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat cerebrum. The section was incubated with ab279649 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
Immunocytochemistry - Anti-BLBP antibody (ab279649)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized rat primary neural/glia cells labelling BLBP with ab279649 at 1/250 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing positive staining in rat primary gliocyte. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection is observed. Anti-Glial Fibrillary Acidic Protein (GFAP) mouse monoclonal antibody was used to counterstain tubulin at 1/100 dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at a 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).
Negative control 1: ab279649 at a 1/250 dilution followed by ab150120 at a 1/1000 dilution.
Negative control 2: Anti-Glial Fibrillary Acidic Protein (GFAP) mouse monoclonal antibody at a 1/100 dilution followed by ab150077 at a 1/1000 dilution.
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Flow Cytometry - Anti-BLBP antibody (ab279649)Flow cytometric analysis of 4% paraformaldehyde fixed 0.1% Tween-20 permeabilized rat primary neural/glia cells labeling BLBP with ab279649 at 1/500 dilution (Right panel) compared with Rabbit monoclonal IgG Isotype Control (ab172730) (Left panel). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077 at 1/2000 dilution was used as the secondary antibody.
