Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Tuesday March 02, 2021

Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3098] (ab133261)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3098] to RUNX1 / AML1 + RUNX3 + RUNX2
Suitable for: WB, IHC-P
Reacts with: Mouse, Human

Product name

Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3098]
See all RUNX1 / AML1+RUNX3+RUNX2 primary antibodies
Description

Rabbit monoclonal [EPR3098] to RUNX1 / AML1 + RUNX3 + RUNX2
Host species

Rabbit
Specificity

The immunogen of this antibody is 100% homologous to mouse RUNX2 sequence.
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human RUNX1/ AML1 aa 150-250. The exact sequence is proprietary.
Database link: Q01196
Positive control
- Molt 4 cell lysate, Human fetal thymus tissue lysate, Mouse thymus tissue lysate, Human tonsil tissue.
General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR3098
Isotype

IgG
Research areas

Western blot - Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3098] (ab133261)

RUNX2 recombinant protein full length, with N-terminal HIS tag, expressed in E.Coli.
RUNX3 overexpression and empty vector control lysates created in HEK293T cells. The protein contains a C-terminal DDK tag.
Western blot - Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3098] (ab133261)

All lanes : Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3098] (ab133261) at 1/1000 dilution

Lane 1 : Molt 4 cell lysate
Lane 2 : Human fetal thymus lysate
Lane 3 : Mouse thymus lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size: 48 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3098] (ab133261)

Immunohistochemical analysis of paraffin embedded Human tonsil tissue labelling RUNX1 / AML1 with ab133261 at 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3098] (ab133261)

Immunohistochemical analysis of paraffin embedded normal Human spleen tissue using ab133261 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3098] (ab133261)

Immunohistochemical analysis of paraffin embedded normal Human testis tissue using ab133261 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3098] (ab133261)

Immunohistochemical analysis of paraffin embedded normal Human heart tissue using ab133261 showing -ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3098] (ab133261)

Immunohistochemical analysis of paraffin embedded normal Human colon tissue using ab133261 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3098] (ab133261)

Immunohistochemical analysis of paraffin embedded normal Human breast tissue using ab133261 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3098] (ab133261)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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