Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] - BSA and Azide free (ab220117)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3099] to RUNX1 / AML1 + RUNX3 + RUNX2 - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] - BSA and Azide free

  • Description

    Rabbit monoclonal [EPR3099] to RUNX1 / AML1 + RUNX3 + RUNX2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IF, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab177141)

  • Positive control

    • WB: MOLT4 cell lysate, fetal thymus tissue lysate. IHC: Human tonsil tissue. IP: MOLT4 cell lysate

  • General notes

    Ab220117 is the carrier-free version of ab92336. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab220117 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Images

  • Western blot - Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] - BSA and Azide free (ab220117)
    Western blot - Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] - BSA and Azide free (ab220117)
    All lanes : Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] - BSA and Azide free (ab220117) at 1/20000 dilution (purified)

    Lane 1 : Raw264.7 ( Mouse Abelson murine leukemia virus-induced tumor macrophage )whole cell lysate
    Lane 2 : MOLT-4 ( Human lymphoblastic leukemia T lymphoblast ) whole cell lysate
    Lane 3 : WEHI-3 ( Mouse leukemia lymphoblast ) whole cell lysate
    Lane 4 : Mouse thymus lysate
    Lane 5 : CTLL-2 (Mouse T lymphocyte ) whole cell lysate
    Lane 6 : Rat thymus lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 49 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

  • Western blot - Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] - BSA and Azide free (ab220117)
    Western blot - Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] - BSA and Azide free (ab220117)
    All lanes : Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] (ab92336) at 1.28 µg/ml (purified)

    Lane 1 : Mouse spleen lysate
    Lane 2 : Mouse thymus lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml

    Predicted band size: 49 kDa



    Blocking/Diluting buffer and concentration: 5% NFDM /TBST

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92336).

  • Immunoprecipitation - Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] - BSA and Azide free (ab220117)
    Immunoprecipitation - Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] - BSA and Azide free (ab220117)

    ab92336 (purified) at 1/50 immunoprecipitating RUNX1 / AML1 + RUNX3 + RUNX2 in 10 μg Molt-4 (Human lymphoblastic leukemia T lymphoblast）whole cell lysate (Lanes 1 and 2, observed at 49 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730) instead of ab92336 in Molt-4 whole cell lysate. For western blotting, ab220117 at 1/500 and HRP Veriblot for IP (ab131366) was used for detection at 1/1000 dilution.

    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92336).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] - BSA and Azide free (ab220117)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] - BSA and Azide free (ab220117)

    Immunohistochemistry staining of RUNX1 / AML1 in formalin-fixed, paraffin-embedded Human tonsil tissue using 1/100 ab92336.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92336).

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] - BSA and Azide free (ab220117)
    Flow Cytometry - Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] - BSA and Azide free (ab220117)
    Flow cytometric analysis of permeabilized Molt-4 cells using anti-RUNX1 ab92336 (red) or a rabbit IgG (negative) (green).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92336).

  • Immunocytochemistry/ Immunofluorescence - Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] - BSA and Azide free (ab220117)
    Immunocytochemistry/ Immunofluorescence - Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] - BSA and Azide free (ab220117) This image is courtesy of an anonymous Abreview.
    ab92336 staining RUNX1 / AML1 in human glioblastoma cell line by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde, permeabilized using 0,1% Triton X 100 in PBS, blocked with 0.5% BSA for 30 minutes at room temperature and then incubated with ab92336 at a 1/50 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/400 dilution. Nuclei are counterstained with DAPI.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92336).

  • Immunocytochemistry/ Immunofluorescence - Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] - BSA and Azide free (ab220117)
    Immunocytochemistry/ Immunofluorescence - Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] - BSA and Azide free (ab220117) This image is courtesy of an anonymous Abreview.
    ab92336 staining RUNX1 / AML1 in rat glioblastoma cell line C6 by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde, permeabilized using 0,1% Triton X 100 in PBS, blocked with 0.5% BSA for 30 minutes at room temperature and then incubated with ab92336 at a 1/50 dilution for 16 hours at 4°C. The secondary used was a Cy3 conjugated goat anti-rabbit polyclonal used at a 1/400 dilution. Nuclei are counterstained with DAPI.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92336).

  • Immunoprecipitation - Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] - BSA and Azide free (ab220117)
    Immunoprecipitation - Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] - BSA and Azide free (ab220117)

    Lane 1 (input): MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysate, 10μg
    Lane 2 (+): MOLT-4 whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab220117 in MOLT-4 whole cell lysate

    Ab220117 Immunoprecipitating RUNX1 / AML1 + RUNX3 + RUNX2 in MOLT-4 whole cell lysates. For western blotting, primary antibody used was ab220117 at 1:500 dilution (1.98 µg/ml). Ab131366 VeriBlot for IP (HRP) was used for detection at 1:1000 dilution.

    Blocking and diluting buffer: 5% NFDM/TBST.

  • Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] - BSA and Azide free (ab220117)
    Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] - BSA and Azide free (ab220117)

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