Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-2B4 antibody [EPR23692-33] - BSA and Azide free (ab275892)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23692-33] to 2B4 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-2B4 antibody [EPR23692-33] - BSA and Azide free
    See all 2B4 primary antibodies

  • Description

    Rabbit monoclonal [EPR23692-33] to 2B4 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: NK-92, KG-1a and THP-1 whole cell lysates; Human spleen tissue lysate. IHC-P: Human tonsil and non-Hodgkin's lymphoma tissue.

  • General notes

    ab275892 is the carrier-free version of ab256370. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab275892 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-2B4 antibody [EPR23692-33] - BSA and Azide free (ab275892)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-2B4 antibody [EPR23692-33] - BSA and Azide free (ab275892)

    This data was developed using ab256370, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human non-Hodgkin's lymphoma tissue labeling 2B4 with ab256370 at 1/50 (12.04 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining in human non-Hodgkin's lymphoma (PMID: 19499526). The section was incubated with ab256370 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-2B4 antibody [EPR23692-33] - BSA and Azide free (ab275892)
    Western blot - Anti-2B4 antibody [EPR23692-33] - BSA and Azide free (ab275892)
    All lanes : Anti-2B4 antibody [EPR23692-33] (ab256370) at 1/1000 dilution

    Lane 1 : NK-92 (human malignant non-Hodgkins lymphoma natural killer cell) whole cell lysate
    Lane 2 : NK-92 whole cell lysate treated with PNGase F
    Lane 3 : A549 (human lung carcinoma epithelial cell) whole cell lysate
    Lane 4 : THP-1 (human monocytic leukemia monocyte) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

    Predicted band size: 42 kDa
    Observed band size: 37,42,70-100 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab256370, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 19499526, 11099315, 16177062, 28126968).

    Negative control: A549 (PMID: 23192659 and Human Protein Atlas).

    Exposure time: Lanes 1-2: 37 seconds; Lanes 3-4: 125 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-2B4 antibody [EPR23692-33] - BSA and Azide free (ab275892)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-2B4 antibody [EPR23692-33] - BSA and Azide free (ab275892)

    This data was developed using ab256370, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling 2B4 with ab256370 at 1/50 (12.04 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining in human tonsil (PMID:19499526). The section was incubated with ab256370 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-2B4 antibody [EPR23692-33] - BSA and Azide free (ab275892)
    Western blot - Anti-2B4 antibody [EPR23692-33] - BSA and Azide free (ab275892)
    Anti-2B4 antibody [EPR23692-33] (ab256370) at 1/1000 dilution + Human spleen tissue lysate at 20 µg

    Secondary
    VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

    Predicted band size: 42 kDa
    Observed band size: 70 kDa why is the actual band size different from the predicted?



    This data was developed using ab256370, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate.

    Exposure time: 48 seconds.

  • Western blot - Anti-2B4 antibody [EPR23692-33] - BSA and Azide free (ab275892)
    Western blot - Anti-2B4 antibody [EPR23692-33] - BSA and Azide free (ab275892)
    Anti-2B4 antibody [EPR23692-33] (ab256370) at 1/1000 dilution + KG-1a (human bone marrow myeloblast) whole cell lysate at 10 µg

    Secondary
    VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/5000 dilution

    Predicted band size: 42 kDa
    Observed band size: 70 kDa why is the actual band size different from the predicted?



    This data was developed using ab256370, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate.

    Exposure time: 24 seconds.

  • Anti-2B4 antibody [EPR23692-33] - BSA and Azide free (ab275892)
    Anti-2B4 antibody [EPR23692-33] - BSA and Azide free (ab275892)

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