Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SHANK2 antibody [EPR24142-35] - BSA and Azide free (ab277532)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24142-35] to SHANK2 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-SHANK2 antibody [EPR24142-35] - BSA and Azide free
    See all SHANK2 primary antibodies

  • Description

    Rabbit monoclonal [EPR24142-35] to SHANK2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-Fr or IP

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse hippocampus tissue lysate; rat brain and hippocampus tissue lysates. IHC-P: Mouse cerebrum and hippocampus tissue; Rat liver and hippocampus tissue.

  • General notes

    ab277532 is the carrier-free version of ab259966.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-SHANK2 antibody [EPR24142-35] - BSA and Azide free (ab277532)
    Western blot - Anti-SHANK2 antibody [EPR24142-35] - BSA and Azide free (ab277532)
    All lanes : Anti-SHANK2 antibody [EPR24142-35] (ab259966) at 1/1000 dilution

    Lane 1 : Mouse hippocampus tissue lysate
    Lane 2 : Rat brain tissue lysate
    Lane 3 : Rat hippocampus tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 159 kDa
    Observed band size: 165 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab259966, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Shank2 is expressed predominately in brain tissue what has described in the literature. (PMID: 9742101).

    Exposure time: 3 minutes.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK2 antibody [EPR24142-35] - BSA and Azide free (ab277532)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK2 antibody [EPR24142-35] - BSA and Azide free (ab277532)

    This data was developed using ab259966, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling SHANK2 with ab259966 at 1/5000 (0.0924 µg/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Cytoplasmic stainig on mouse cerebrum (PMID:29572432, 28179641). The section was incubated with ab259966 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK2 antibody [EPR24142-35] - BSA and Azide free (ab277532)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK2 antibody [EPR24142-35] - BSA and Azide free (ab277532)

    This data was developed using ab259966, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling SHANK2 with ab259966 at 1/5000 (0.0924 µg/m) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Cytoplasmic stainig on mouse hippocampus (PMID: 27581745, 29572432, 28179641). The section was incubated with ab259966 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK2 antibody [EPR24142-35] - BSA and Azide free (ab277532)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK2 antibody [EPR24142-35] - BSA and Azide free (ab277532)

    This data was developed using ab259966, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling SHANK2 with ab259966 at 1/5000 (0.0924 µg/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on bile duct in rat liver (PMID: 14977424). The section was incubated with ab259966 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK2 antibody [EPR24142-35] - BSA and Azide free (ab277532)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK2 antibody [EPR24142-35] - BSA and Azide free (ab277532)

    This data was developed using ab259966, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labeling SHANK2 with ab259966 at 1/5000 (0.0924 µg/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Cytoplasmic stainig on rat hippocampus. The section was incubated with ab259966 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-SHANK2 antibody [EPR24142-35] - BSA and Azide free (ab277532)
    Anti-SHANK2 antibody [EPR24142-35] - BSA and Azide free (ab277532)

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