Anti-AP-2 complex subunit alpha-1 antibody (ab189995)
Key features and details
- Goat polyclonal to AP-2 complex subunit alpha-1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-AP-2 complex subunit alpha-1 antibody
See all AP-2 complex subunit alpha-1 primary antibodies -
Description
Goat polyclonal to AP-2 complex subunit alpha-1 -
Host species
Goat -
Specificity
ab189995 is expected to recognize reported isoform 1 (NP_055018.2) only. -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cat, Dog, Pig, Chimpanzee, Gorilla
-
Immunogen
Synthetic peptide corresponding to Human AP-2 complex subunit alpha-1 aa 700-800 (internal sequence) (Cysteine residue). NP_055018.2.
Database link: O95782 -
Positive control
- Human brain cortex tissue; HeLa cells, stably expressing a chimeric construct including the brain-specific splice variant of Mouse AP-2 complex subunit alpha-1; Mouse brain lysate and Human Frontal Cortex lysate.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-AP-2 complex subunit alpha-1 antibody (ab189995)Anti-AP-2 complex subunit alpha-1 antibody (ab189995) at 0.01 µg/ml + Human Frontal Cortex lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 108 kDa
-
Western blot - Anti-AP-2 complex subunit alpha-1 antibody (ab189995)Lanes 1-3 : total protein blot stained with Ponceau Red
Lanes 4-6 : Anti-AP-2 complex subunit alpha-1 antibody (ab189995) at 0.1 µg/ml
Lanes 1 & 4 : Mouse Liver lysate
Lanes 2 & 5 : Mouse Brain lysate
Lanes 3 & 6 : Mouse Kidney lysate
Lysates/proteins at 5 µg per lane.
Developed using the ECL technique.
Predicted band size: 108 kDaThe left panel is stained with Ponceau red.
-
Immunocytochemistry/ Immunofluorescence - Anti-AP-2 complex subunit alpha-1 antibody (ab189995)Immunofluorescent analysis of methanol-fixed HeLa cells stably expressing a chimeric construct including the brain-specific splice variant of Mouse AP-2 complex subunit alpha-1 labeling AP-2 complex subunit alpha-1 with ab189995 at 0.1 µg/ml.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-2 complex subunit alpha-1 antibody (ab189995)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain cortex tissue labeling AP-2 complex subunit alpha-1 with ab189995 at 5µg/ml.
