Anti-AP-2 complex subunit alpha-1 antibody (ab3707)
Key features and details
- Goat polyclonal to AP-2 complex subunit alpha-1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-AP-2 complex subunit alpha-1 antibody
See all AP-2 complex subunit alpha-1 primary antibodies -
Description
Goat polyclonal to AP-2 complex subunit alpha-1 -
Host species
Goat -
Specificity
The immunogen sequence is found in both AP2A2 and AP2A1. This antibody recognises a band of just under 100kD in multiple human cell lines (see picture), the band can be blocked with the immunising peptide. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human AP-2 complex subunit alpha-1 aa 3-14.
Sequence:AVSKGDGMRGLAC
(Peptide available asab11698)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-AP-2 complex subunit alpha-1 antibody (ab3707)
Western blot using ab3707 at 1/500.
Lane 1: HeLa Nuclear Extract
Lane 2: HeLa Whole Cell Lysate
Lane 3: 293 Whole Cell Lysate
Lane 4: A431 Whole Cell Lysate
Lane 5: Jurkat Whole Cell Lysate
Lane 6: HeLa Nuclear Extract + blocking/immunising peptide
Lane 7: HeLa Whole Cell Lysate + blocking/immunising peptide
Lane 8: 293 Whole Cell Lysate + blocking/immunising peptide
Lane 9: A431 Whole Cell Lysate + blocking/immunising peptide
Lane 10: Jurkat Whole Cell Lysate + blocking/immunising peptide
AP2 alpha proteins (AP2A2 and AP2A1) have predicted molecular weights of 104 and 107kD. The band at just below 97kD (that is blocked by the immunising peptide) represents AP2 alpha, the lower bands are not blocked completely by the immunising peptide and are believed to be non-specific.
Secondary ab: Rabbit polyclonal to Goat IgG HRP
ab6741 (1/5000)
Exposure time: 1 minute.
