Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-AP-2 complex subunit alpha-1 antibody (ab3707)

Key features and details

  • Goat polyclonal to AP-2 complex subunit alpha-1
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-AP-2 complex subunit alpha-1 antibody
    See all AP-2 complex subunit alpha-1 primary antibodies

  • Description

    Goat polyclonal to AP-2 complex subunit alpha-1

  • Host species

    Goat

  • Specificity

    The immunogen sequence is found in both AP2A2 and AP2A1. This antibody recognises a band of just under 100kD in multiple human cell lines (see picture), the band can be blocked with the immunising peptide.

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human AP-2 complex subunit alpha-1 aa 3-14.
    Sequence:

    AVSKGDGMRGLAC


    (Peptide available as ab11698)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-AP-2 complex subunit alpha-1 antibody (ab3707)
    Western blot - Anti-AP-2 complex subunit alpha-1 antibody (ab3707)

    Western blot using ab3707 at 1/500.

    Lane 1: HeLa Nuclear Extract
    Lane 2: HeLa Whole Cell Lysate
    Lane 3: 293 Whole Cell Lysate
    Lane 4: A431 Whole Cell Lysate
    Lane 5: Jurkat Whole Cell Lysate
    Lane 6: HeLa Nuclear Extract + blocking/immunising peptide
    Lane 7: HeLa Whole Cell Lysate + blocking/immunising peptide
    Lane 8: 293 Whole Cell Lysate + blocking/immunising peptide
    Lane 9: A431 Whole Cell Lysate + blocking/immunising peptide
    Lane 10: Jurkat Whole Cell Lysate + blocking/immunising peptide

    AP2 alpha proteins (AP2A2 and AP2A1) have predicted molecular weights of 104 and 107kD. The band at just below 97kD (that is blocked by the immunising peptide) represents AP2 alpha, the lower bands are not blocked completely by the immunising peptide and are believed to be non-specific.

    Secondary ab: Rabbit polyclonal to Goat IgG HRP
    ab6741 (1/5000)
    Exposure time: 1 minute.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-AP-2 complex subunit alpha-1 antibody (ab3707)

© 2021 Astana Biomed Group LLP. All rights reserved.