Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Telomerase reverse transcriptase antibody [Y182] (ab32020)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y182] to Telomerase reverse transcriptase
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Telomerase reverse transcriptase antibody [Y182]
    See all Telomerase reverse transcriptase primary antibodies

  • Description

    Rabbit monoclonal [Y182] to Telomerase reverse transcriptase

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Telomerase reverse transcriptase aa 1100-1200 (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: HeLa, HEK-293, Jurkat, SK-BR-3, HL60, MCF7, PC-3 and K-562 cell lysates. IP: HeLa whole cell lysate.

  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Telomerase reverse transcriptase antibody [Y182] (ab32020)
    Western blot - Anti-Telomerase reverse transcriptase antibody [Y182] (ab32020)
    All lanes : Anti-Telomerase reverse transcriptase antibody [Y182] (ab32020) at 1/1000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates
    Lane 3 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates
    Lane 4 : SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysates
    Lane 5 : HL-60 (Human acute promyelocytic leukemia promyeloblast) whole cell lysates
    Lane 6 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
    Lane 7 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysates
    Lane 8 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 127 kDa
    Observed band size: 127 kDa

  • Immunoprecipitation - Anti-Telomerase reverse transcriptase antibody [Y182] (ab32020)
    Immunoprecipitation - Anti-Telomerase reverse transcriptase antibody [Y182] (ab32020)

    ab32020 (purified) at 1:100 dilution (2µg) immunoprecipitating Telomerase reverse transcriptase in HeLa whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab32020 & HeLa whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32020 in HeLa whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-Telomerase reverse transcriptase antibody [Y182] (ab32020)
    Western blot - Anti-Telomerase reverse transcriptase antibody [Y182] (ab32020)
    Anti-Telomerase reverse transcriptase antibody [Y182] (ab32020) at 1/1000 dilution + Hela (human epithelial cell line from cervix adenocarcinoma) cell lysate

    Predicted band size: 127 kDa
    Observed band size: 122 kDa
    why is the actual band size different from the predicted?

  • Anti-Telomerase reverse transcriptase antibody [Y182] (ab32020)
    Anti-Telomerase reverse transcriptase antibody [Y182] (ab32020)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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