Antibodies, Proteins, Kits and Reagents for Life Science

361 843 ₸ Product size

100 µl 361 843 ₸ 10 µl 144 067 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Telomerase reverse transcriptase antibody [Y182] (ab32020)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [Y182] to Telomerase reverse transcriptase
Suitable for: WB, IP
Reacts with: Human

Product name

Anti-Telomerase reverse transcriptase antibody [Y182]
See all Telomerase reverse transcriptase primary antibodies
Description

Rabbit monoclonal [Y182] to Telomerase reverse transcriptase
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human Telomerase reverse transcriptase aa 1100-1200 (C terminal). The exact sequence is proprietary.
Positive control
- WB: HeLa, HEK-293, Jurkat, SK-BR-3, HL60, MCF7, PC-3 and K-562 cell lysates. IP: HeLa whole cell lysate.
General notes
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
IP	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol
Concentration information loading...
Purity

Protein A purified
Purification notes

Cells supernatant
Clonality

Monoclonal
Clone number

Y182
Isotype

IgG
Research areas

Western blot - Anti-Telomerase reverse transcriptase antibody [Y182] (ab32020)

All lanes : Anti-Telomerase reverse transcriptase antibody [Y182] (ab32020) at 1/1000 dilution (Purified)

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates
Lane 3 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates
Lane 4 : SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lane 5 : HL-60 (Human acute promyelocytic leukemia promyeloblast) whole cell lysates
Lane 6 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lane 7 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysates
Lane 8 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 127 kDa
Observed band size: 127 kDa
Immunoprecipitation - Anti-Telomerase reverse transcriptase antibody [Y182] (ab32020)

ab32020 (purified) at 1:100 dilution (2µg) immunoprecipitating Telomerase reverse transcriptase in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab32020 & HeLa whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32020 in HeLa whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
Western blot - Anti-Telomerase reverse transcriptase antibody [Y182] (ab32020)

Anti-Telomerase reverse transcriptase antibody [Y182] (ab32020) at 1/1000 dilution + Hela (human epithelial cell line from cervix adenocarcinoma) cell lysate

Predicted band size: 127 kDa
Observed band size: 122 kDa
why is the actual band size different from the predicted?
Anti-Telomerase reverse transcriptase antibody [Y182] (ab32020)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com