Anti-Telomerase reverse transcriptase antibody (ab230527)
Key features and details
- Rabbit polyclonal to Telomerase reverse transcriptase
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Telomerase reverse transcriptase antibody
See all Telomerase reverse transcriptase primary antibodies -
Description
Rabbit polyclonal to Telomerase reverse transcriptase -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Telomerase reverse transcriptase aa 600-700 conjugated to keyhole limpet haemocyanin.
Database link: O14746 -
Positive control
- WB: Jurkat whole cell lysate (ab7899). IHC-P: Human skin tissue. ICC/IF: SH-SY5Y and HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Ammonium Sulphate Precipitation -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Telomerase reverse transcriptase antibody (ab230527)Anti-Telomerase reverse transcriptase antibody (ab230527) at 1/1000 dilution + Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 127 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Telomerase reverse transcriptase antibody (ab230527)Formalin-fixed, paraffin-embedded human skin tissue stained for Telomerase reverse transcriptase with ab230527 at 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-Telomerase reverse transcriptase antibody (ab230527)4% PFA-fixed, 0.2% Triton X-100 permeabilized SH-SY5Y (human neuroblastoma cell line from bone marrow) cells stained forTelomerase reverse transcriptase (green) using ab230527 at 1/200 dilution followed by Alexa Fluor® 488-congugated Donkey Anti-Rabbit IgG at 1/1000 dilution in ICC/IF.
The nuclear counter stain is Hoechst 33342 (blue).
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Immunocytochemistry/ Immunofluorescence - Anti-Telomerase reverse transcriptase antibody (ab230527)4% PFA-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Telomerase reverse transcriptase (green) using ab230527 at 1/25 dilution followed by Alexa Fluor® 488-congugated Donkey Anti-Rabbit IgG at 1/400 dilution in ICC/IF.
The nuclear counter stain is DAPI (blue). Cytoplasmic actin is counterstained with Alexa Fluor 555® conjugated Phalloidin (red).
