Human EGFL7 ELISA Kit (ab275896)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.11 ng/ml
- Range: 0.47 ng/ml - 30 ng/ml
- Sample type: Cell culture media, Cell Lysate, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human EGFL7 ELISA Kit
See all EGFL7 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Extract 8 7.5% Inter-assay Sample n Mean SD CV% Extract 4 9.3% -
Sample type
Serum, Cell Lysate, Cell culture media, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.11 ng/ml -
Range
0.47 ng/ml - 30 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 73 70% - 76% Cell Lysate 81 80% - 82% Cell culture media 95 94% - 98% EDTA Plasma 79 78% - 79% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human EGFL7 ELISA kit (ab275896) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human EGFL7 protein in human serum, plasma (EDTA), cell culture supernatant, and cell and tissue extract. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human EGFL7 with 0.11 ng/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Epidermal growth factor-like protein 7 (EGFL7), also known as Vascular endothelial statin, NOTCH4-like protein, and Zneu1, is a protein encoded by the gene EGFL7. EGFL7 is expressed as a 273-amino acid protein containing a 22- amino acid N-terminal signal peptide, a 78- amino acid EMI domain, and two EGF-like domains. EGFL7 helps regulate vascular tubulogenesis, inhibits platelet-derived growth factor BB-induced smooth muscle migration, and promotes endothelial cell adhesion to the extracellular matrix.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human EGFL7 Capture Antibody 1 x 600µl 10X Human EGFL7 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human EGFL7 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Regulates vascular tubulogenesis in vivo. Inhibits platelet-derived growth factor (PDGF)-BB-induced smooth muscle cell migration and promotes endothelial cells adhesion to the substrate in vitro. -
Sequence similarities
Contains 2 EGF-like domains.
Contains 1 EMI domain. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- EGF like domain 7
- EGF like domain containing protein 7
- EGF like domain multiple 7
see all -
Database links
- Entrez Gene: 51162 Human
- Omim: 608582 Human
- SwissProt: Q9UHF1 Human
- Unigene: 91481 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human EGFL7 standard curve in Sample Diluent NS.The EGFL7 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native and spiked EGFL7 in Huvec extract based on a 300 µg/mL extract load.Huvec extract was spiked with recombinant EGFL7 protein alongside due to the low native expression of the target protein. The concentrations of EGFL7 were measured in duplicate and interpolated from the EGFL7 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Sandwich ELISA - Human EGFL7 ELISA Kit (ab275896)To learn more about the advantages of recombinant antibodies see here.
