Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Telomerase reverse transcriptase antibody [Y182] - BSA and Azide free (ab181830)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y182] to Telomerase reverse transcriptase - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Telomerase reverse transcriptase antibody [Y182] - BSA and Azide free
    See all Telomerase reverse transcriptase primary antibodies

  • Description

    Rabbit monoclonal [Y182] to Telomerase reverse transcriptase - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Telomerase reverse transcriptase aa 1100-1200 (C terminal). The exact sequence is proprietary.

  • Positive control

    • IP: HeLa cells.

  • General notes

    Ab181830 is the carrier-free version of ab32020. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab181830 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunoprecipitation - Anti-Telomerase reverse transcriptase antibody [Y182] - BSA and Azide free (ab181830)
    Immunoprecipitation - Anti-Telomerase reverse transcriptase antibody [Y182] - BSA and Azide free (ab181830)

    ab32020 (purified) at 1:100 dilution (2µg) immunoprecipitating Telomerase reverse transcriptase in HeLa whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab32020 & HeLa whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32020 in HeLa whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Anti-Telomerase reverse transcriptase antibody [Y182] - BSA and Azide free (ab181830)
    Anti-Telomerase reverse transcriptase antibody [Y182] - BSA and Azide free (ab181830)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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