Anti-Telomerase reverse transcriptase antibody [Y182] - BSA and Azide free (ab181830)
![Anti-Telomerase reverse transcriptase antibody [Y182] - BSA and Azide free (ab181830)](https://www.abcam.com/ps/products/181/ab181830/Images/ab181830-337464-anti-telomerase-reverse-transcriptase-antibody-y182-immunoprecipitation.jpg "Anti-Telomerase reverse transcriptase antibody [Y182] - BSA and Azide free (ab181830)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y182] to Telomerase reverse transcriptase - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-Telomerase reverse transcriptase antibody [Y182] - BSA and Azide free
See all Telomerase reverse transcriptase primary antibodies -
Description
Rabbit monoclonal [Y182] to Telomerase reverse transcriptase - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP Human
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Immunogen
Synthetic peptide within Human Telomerase reverse transcriptase aa 1100-1200 (C terminal). The exact sequence is proprietary.
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Positive control
- IP: HeLa cells.
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General notes
Ab181830 is the carrier-free version of ab32020. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab181830 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Y182 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-Telomerase reverse transcriptase antibody [Y182] - BSA and Azide free (ab181830)
ab32020 (purified) at 1:100 dilution (2µg) immunoprecipitating Telomerase reverse transcriptase in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab32020 & HeLa whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32020 in HeLa whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
![Anti-Telomerase reverse transcriptase antibody [Y182] - BSA and Azide free (ab181830)](https://www.abcam.com/ps/products/181/ab181830/Images/ab181830-3-benefits-of-recombinant-antibodies.png)
Anti-Telomerase reverse transcriptase antibody [Y182] - BSA and Azide free (ab181830)
