Anti-DKC1/Dyskerin antibody (ab93777)
Key features and details
- Rabbit polyclonal to DKC1/Dyskerin
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DKC1/Dyskerin antibody
See all DKC1/Dyskerin primary antibodies -
Description
Rabbit polyclonal to DKC1/Dyskerin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human DKC1/Dyskerin aa 340-390.
Database link: NP_001354.1 -
General notes
This product was previously labelled as DKC1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-DKC1/Dyskerin antibody (ab93777)All lanes : Anti-DKC1/Dyskerin antibody (ab93777) at 0.04 µg/ml
Lane 1 : HeLa cell lysate at 50 µg
Lane 2 : HeLa cell lysate at 15 µg
Lane 3 : HeLa cell lysate at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 58 kDa
Exposure time: 3 minutes
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Immunoprecipitation - Anti-DKC1/Dyskerin antibody (ab93777)Detection of Human DKC1/Dyskerin by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded)using ab93777 at 10 ug/mg lysate (LANE 1). DKC1/Dyskerin was also immunoprecipitated by an antibody which recognizes a downstream epitope (LANE 2). Lane 3 represents IgG Ip control. subsequent WB detection was performed using 1 ug/ml ab93777. Detection: Chemiluminescence with exposure times of 10 seconds.
All lanes : Anti-DKC1/Dyskerin antibody (ab93777) at 1 µg/ml
Lane 1 : HeLa cell lysate immunoprecipitated with ab 93777 at 10ug/mg lysate.
Lane 2 : HeLa cell lysate immunoprecipitated with anti-DKC1/Dyskerinantibody at 10ug/mg lysate.
Lane 3 : HeLa cell lysate immunoprecipitated with control IgG at 10ug/mg lysate.
Lysates/proteins at 200 µg per lane.
Developed using the ECL technique.
Exposure time: 10 seconds
