Anti-SREBP1 antibody [2A4] (ab3259)
![Anti-SREBP1 antibody [2A4] (ab3259)](https://www.abcam.com/ps/products/3/ab3259/Images/ab3259-347647-ab3259-clone-2A4-AP4511748-western-blot-HeLa-HAP1.jpg "Anti-SREBP1 antibody [2A4] (ab3259)")
Key features and details
- Mouse monoclonal [2A4] to SREBP1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-SREBP1 antibody [2A4]
See all SREBP1 primary antibodies -
Description
Mouse monoclonal [2A4] to SREBP1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanWB Human
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Immunogen
Recombinant fragment (His-tag) within Human SREBP1 aa 300-450 (N terminal). The exact sequence is proprietary.
Database link: P36956 -
Epitope
Amino acids 301 - 407. -
Positive control
- WB: HeLa and HAP1 whole cell lysates.
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General notes
This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
2A4 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
- Epigenetics and Nuclear Signaling
- Transcription
- Domain Families
- HLH / Leucine Zipper
- HLH / Leucine Zipper
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Images
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Western blot - Anti-SREBP1 antibody [2A4] (ab3259)All lanes : Anti-SREBP1 antibody [2A4] (ab3259) at 5 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : HAP1 whole cell lysate
Lysates/proteins at 40 µg per lane.
Performed under reducing conditions.
Predicted band size: 127 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?
Additional bands at: 65 kDa (possible cleavage fragment)This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was blocked for an hour using 5% milk before ab3259 and ab181602 (rabbit anti-GAPDH loading control) were incubated overnight at 4°C at a 5ug/ml concentration and 1/20000 dilution respectively. Antibody binding was detected using Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
