Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸

Order now and get it on Wednesday March 10, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20954] to RON
Suitable for: WB, IHC-P
Reacts with: Human

Product name

Anti-RON antibody [EPR20954]
See all RON primary antibodies
Description

Rabbit monoclonal [EPR20954] to RON
Host species

Rabbit
Specificity

ab227979 detects a distinct immunogen compared to ab227980.
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: BxPC-3, SK-BR-3, SW480 and HCT 116 whole cell lysates; Human stomach tissue lysate. IHC-P: Human stomach, tonsil and bladder cancer tissues.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20954
Isotype

IgG
Research areas

Western blot - Anti-RON antibody [EPR20954] (ab227979)

Lanes 1-4 : Anti-RON antibody [EPR20954] (ab227979) at 1/5000 dilution
Lane 5 : Anti-RON antibody [EPR20954] (ab227979) at 1/1000 dilution

Lane 1 : BxPC-3 (human pancreas adenocarcinoma cell line) whole cell lysate
Lane 2 : SK-BR-3 (human mammary gland adenocarcinoma cell line) whole cell lysate
Lane 3 : SW480 (human colorectal adenocarcinoma cell line) whole cell lysate
Lane 4 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate
Lane 5 : Human stomach tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 152 kDa
Observed band size: 100, 152 kDa
why is the actual band size different from the predicted?

Exposure times: Lane 1: 10 seconds; Lane 2: 58 seconds; Lanes 3,5: 3 minutes; Lane 4: 2 minutes.

Blocking/Dilution buffer: 5% NFDM/TBST.

The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.

The 185kDa RON precursor protein is cleaved into a 40kDa α and 150kDa β chain, which are linked by disulphide bonds in the 180kDa mature form (PMID: 9890710, PMID: 23792360, PMID: 17889431). There are several isoforms of RON, including a 110kDa truncation (23792360). ab227979 is specific for the β chain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RON antibody [EPR20954] (ab227979)

Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling RON with ab227979 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Cytoplasmic and membranous staining in human gastric epithelium is observed (PMID: 9890710). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RON antibody [EPR20954] (ab227979)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling RON with ab227979 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Cytoplasmic and membranous staining in germinal centers of human tonsil is observed (PMID: 9890710). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RON antibody [EPR20954] (ab227979)

Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling RON with ab227979 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Membranous staining on human bladder cancer cells is observed (PMID: 15870710). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-RON antibody [EPR20954] (ab227979)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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