Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RON antibody [EPR20954] - BSA and Azide free (ab228853)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20954] to RON - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-RON antibody [EPR20954] - BSA and Azide free
    See all RON primary antibodies

  • Description

    Rabbit monoclonal [EPR20954] to RON - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC: Human stomach tissue.

  • General notes

    Ab228853 is the carrier-free version of ab227979. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab228853 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RON antibody [EPR20954] - BSA and Azide free (ab228853)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RON antibody [EPR20954] - BSA and Azide free (ab228853)

    Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling RON with ab227979 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Membranous staining on human bladder cancer cells is observed (PMID: 15870710). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227979).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RON antibody [EPR20954] - BSA and Azide free (ab228853)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RON antibody [EPR20954] - BSA and Azide free (ab228853)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling RON with ab227979 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Cytoplasmic and membranous staining in germinal centers of human tonsil is observed (PMID: 9890710). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227979).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RON antibody [EPR20954] - BSA and Azide free (ab228853)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RON antibody [EPR20954] - BSA and Azide free (ab228853)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling RON with ab227979 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Cytoplasmic and membranous staining in human gastric epithelium is observed (PMID: 9890710). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227979).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-RON antibody [EPR20954] - BSA and Azide free (ab228853)
    Anti-RON antibody [EPR20954] - BSA and Azide free (ab228853)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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