Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PAK1 (phospho T212) antibody [EPR20045] (ab223852)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20045] to PAK1 (phospho T212)
  • Suitable for: Dot blot, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-PAK1 (phospho T212) antibody [EPR20045]
    See all PAK1 primary antibodies

  • Description

    Rabbit monoclonal [EPR20045] to PAK1 (phospho T212)

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Dot blot: PAK1 (phospho T212) peptide. IHC-P: Human prostate tissue, lung squamous cancer and colon cancer tissue. ICC/IF: HeLa and A549 cells. Flow cytometry: HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Dot Blot - Anti-PAK1 (phospho T212) antibody [EPR20045] (ab223852)
    Dot Blot - Anti-PAK1 (phospho T212) antibody [EPR20045] (ab223852)

    Dot blot analysis of PAK1 (phospho T212) peptide using ab223852 1/1,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (ab97051), was used as secondary antibody at 1/100,000 dilution.

    Lane 1: PAK1 (phospho T212) peptide.

    Lane 2: PAK1 non-phospho peptide.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

     

  • Immunocytochemistry/ Immunofluorescence - Anti-PAK1 (phospho T212) antibody [EPR20045] (ab223852)
    Immunocytochemistry/ Immunofluorescence - Anti-PAK1 (phospho T212) antibody [EPR20045] (ab223852)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling PAK1 (phospho T212) with ab223852 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1,000 dilution (green). Confocal image showing positive staining on HeLa cells in M phase. The signal decreased after treatment with Lambda Protein Phosphatase 31℃ for 2 hours (treated).

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

    The negative control: PBS followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1,000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK1 (phospho T212) antibody [EPR20045] (ab223852)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK1 (phospho T212) antibody [EPR20045] (ab223852)

    Immunohistochemical analysis of paraffin-embedded human lung squamous cancer tissue labeling PAK1 (phospho T212) with ab223852 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on tumor cells of human lung squamous cancer (PMID: 19876919; PMID: 12176334) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

     

  • Flow Cytometry - Anti-PAK1 (phospho T212) antibody [EPR20045] (ab223852)
    Flow Cytometry - Anti-PAK1 (phospho T212) antibody [EPR20045] (ab223852)

    Flow cytometric analysis of 80% methanol-fixed, 0.1% Tween-20 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling PAK1 (phospho T212) with ab223852 at 1/50 (right panel) compared with a Rabbit monoclonal IgG (ab172730) Isotype control (left panel). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2,000 dilution was used as the secondary antibody. Unlabeled control cells without incubation with primary antibody and secondary antibody (Blue).

    Cells were pretreated with 20 μg/ml RNase A for 30 minutes to eliminate the non-specific binding between RNA and PI (Propidium iodide). PAK1 (phospho T212) is highly expressed in mitotic cells (PMID: 12176334).

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK1 (phospho T212) antibody [EPR20045] (ab223852)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK1 (phospho T212) antibody [EPR20045] (ab223852)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling PAK1 (phospho T212) with ab223852 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on tumor cells of human colon cancer (PMID: 19876919; PMID: 12176334) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK1 (phospho T212) antibody [EPR20045] (ab223852)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK1 (phospho T212) antibody [EPR20045] (ab223852)

    Immunohistochemical analysis of paraffin-embedded human prostate tissue labeling PAK1 (phospho T212) with ab223852 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Sporadic nuclear staining on human prostate (PMID: 19876919; PMID: 12176334) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunocytochemistry/ Immunofluorescence - Anti-PAK1 (phospho T212) antibody [EPR20045] (ab223852)
    Immunocytochemistry/ Immunofluorescence - Anti-PAK1 (phospho T212) antibody [EPR20045] (ab223852)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (human lung carcinoma cell line) cells labeling PAK1 (phospho T212) with ab223852 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1,000 dilution (green). Confocal image showing positive staining on HeLa cells in M phase. The signal decreased after treatment with Lambda Protein Phosphatase 31℃ for 2 hours (treated).

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

    The negative control: PBS followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1,000 dilution.

  • Anti-PAK1 (phospho T212) antibody [EPR20045] (ab223852)
    Anti-PAK1 (phospho T212) antibody [EPR20045] (ab223852)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-PAK1 (phospho T212) antibody [EPR20045] (ab223852)

© 2021 Astana Biomed Group LLP. All rights reserved.