Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Tuesday March 02, 2021

Anti-PAK1 (phospho T212) antibody [EPR20045] (ab223852)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20045] to PAK1 (phospho T212)
Suitable for: Dot blot, IHC-P, ICC/IF, Flow Cyt
Reacts with: Human

Product name

Anti-PAK1 (phospho T212) antibody [EPR20045]
See all PAK1 primary antibodies
Description

Rabbit monoclonal [EPR20045] to PAK1 (phospho T212)
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Dot blot: PAK1 (phospho T212) peptide. IHC-P: Human prostate tissue, lung squamous cancer and colon cancer tissue. ICC/IF: HeLa and A549 cells. Flow cytometry: HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20045
Isotype

IgG
Research areas

Dot Blot - Anti-PAK1 (phospho T212) antibody [EPR20045] (ab223852)

Dot blot analysis of PAK1 (phospho T212) peptide using ab223852 1/1,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (ab97051), was used as secondary antibody at 1/100,000 dilution.

Lane 1: PAK1 (phospho T212) peptide.

Lane 2: PAK1 non-phospho peptide.

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 3 minutes.
Immunocytochemistry/ Immunofluorescence - Anti-PAK1 (phospho T212) antibody [EPR20045] (ab223852)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling PAK1 (phospho T212) with ab223852 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1,000 dilution (green). Confocal image showing positive staining on HeLa cells in M phase. The signal decreased after treatment with Lambda Protein Phosphatase 31℃ for 2 hours (treated).

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution (red).

The negative control: PBS followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1,000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK1 (phospho T212) antibody [EPR20045] (ab223852)

Immunohistochemical analysis of paraffin-embedded human lung squamous cancer tissue labeling PAK1 (phospho T212) with ab223852 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on tumor cells of human lung squamous cancer (PMID: 19876919; PMID: 12176334) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Flow Cytometry - Anti-PAK1 (phospho T212) antibody [EPR20045] (ab223852)

Flow cytometric analysis of 80% methanol-fixed, 0.1% Tween-20 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling PAK1 (phospho T212) with ab223852 at 1/50 (right panel) compared with a Rabbit monoclonal IgG (ab172730) Isotype control (left panel). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077), at 1/2,000 dilution was used as the secondary antibody. Unlabeled control cells without incubation with primary antibody and secondary antibody (Blue).

Cells were pretreated with 20 μg/ml RNase A for 30 minutes to eliminate the non-specific binding between RNA and PI (Propidium iodide). PAK1 (phospho T212) is highly expressed in mitotic cells (PMID: 12176334).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK1 (phospho T212) antibody [EPR20045] (ab223852)

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling PAK1 (phospho T212) with ab223852 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on tumor cells of human colon cancer (PMID: 19876919; PMID: 12176334) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK1 (phospho T212) antibody [EPR20045] (ab223852)

Immunohistochemical analysis of paraffin-embedded human prostate tissue labeling PAK1 (phospho T212) with ab223852 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Sporadic nuclear staining on human prostate (PMID: 19876919; PMID: 12176334) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunocytochemistry/ Immunofluorescence - Anti-PAK1 (phospho T212) antibody [EPR20045] (ab223852)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (human lung carcinoma cell line) cells labeling PAK1 (phospho T212) with ab223852 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1,000 dilution (green). Confocal image showing positive staining on HeLa cells in M phase. The signal decreased after treatment with Lambda Protein Phosphatase 31℃ for 2 hours (treated).

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

The negative control: PBS followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1,000 dilution.
Anti-PAK1 (phospho T212) antibody [EPR20045] (ab223852)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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