Anti-PAK1 (phospho S204) antibody (ab79503)
Key features and details
- Rabbit polyclonal to PAK1 (phospho S204)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PAK1 (phospho S204) antibody
See all PAK1 primary antibodies -
Description
Rabbit polyclonal to PAK1 (phospho S204) -
Host species
Rabbit -
Specificity
ab79503 detects endogenous levels of PAK1 only when phosphorylated at serine 204. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Mouse
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Immunogen
Synthetic phosphopeptide derived from human PAK1 around the phosphorylation site of serine 204 (TRSPVI)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified by affinity chromatography using an epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using a non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PAK1 (phospho S204) antibody (ab79503)All lanes : Anti-PAK1 (phospho S204) antibody (ab79503) at 1/1000 dilution
Lane 1 : extracts from 3T3 cells (treated
with UV for 15 mins)
Lane 2 : extracts from 3T3 cells (treated
with UV for 15 mins) with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 61 kDa
Observed band size: 61 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK1 (phospho S204) antibody (ab79503)Immunohistochemistry analysis of paraffin-embedded human brain tissue, stained with ab79503, at 1/100 dilution, in the absence and presence of the immunising peptide
