Anti-PAK1 (phospho S20) antibody [EP655Y] - BSA and Azide free (ab235117)
![Anti-PAK1 (phospho S20) antibody [EP655Y] - BSA and Azide free (ab235117)](https://www.abcam.com/ps/products/235/ab235117/Images/ab235117-319114-172.jpg "Anti-PAK1 (phospho S20) antibody [EP655Y] - BSA and Azide free (ab235117)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP655Y] to PAK1 (phospho S20) - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-PAK1 (phospho S20) antibody [EP655Y] - BSA and Azide free
See all PAK1 primary antibodies -
Description
Rabbit monoclonal [EP655Y] to PAK1 (phospho S20) - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa cell lysate.
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General notes
ab235117 is the carrier-free version of ab51244 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab235117 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP655Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PAK1 (phospho S20) antibody [EP655Y] - BSA and Azide free (ab235117)All lanes : Anti-PAK1 (phospho S20) antibody [EP655Y] (ab51244) at 1/1000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) wole cell lysates with 5% NFDM/TBST
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 100mM calyculin A for 30 minutes whole cell lysates with 5% NFDM/TBST
Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 100mM calyculin A for 30 minutes. Then the membrane was incubated with phosphatase with 5% NFDM/TBST
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 61 kDaThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51244).
We are unable to define the extra bands.
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![Anti-PAK1 (phospho S20) antibody [EP655Y] - BSA and Azide free (ab235117)](https://www.abcam.com/ps/products/235/ab235117/Images/ab235117-2-benefits-of-recombinant-antibodies.png)
Anti-PAK1 (phospho S20) antibody [EP655Y] - BSA and Azide free (ab235117)
