Anti-PAK1 (phospho S199) antibody (ab192814)
Key features and details
- Rabbit polyclonal to PAK1 (phospho S199)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PAK1 (phospho S199) antibody
See all PAK1 primary antibodies -
Description
Rabbit polyclonal to PAK1 (phospho S199) -
Host species
Rabbit -
Specificity
ab192814 detects endogenous levels of PAK1 only when phosphorylated at serine 199. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human PAK1 (phospho S199) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary. Peptide sequence around phosphorylation site of Serine 199 (T-K-S(p)-V-I).
Database link: Q13153 -
Positive control
- Lysate of starved LOVO cells and Human placenta tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.87% Sodium chloride
PBS without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PAK1 (phospho S199) antibody (ab192814)All lanes : Anti-PAK1 (phospho S199) antibody (ab192814) at 1/500 dilution
Lane 1 : Lysate of starved LOVO cells
Lane 2 : Lysate of starved LOVO cells with antigen-specific peptide
Predicted band size: 61 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK1 (phospho S199) antibody (ab192814)Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling PAK1 (phospho S199) using ab192814 at a 1/50 dilution, the right panel was treated with blocking peptide.
