Anti-PAK1 (phospho T212) antibody (ab75599)
Key features and details
- Rabbit polyclonal to PAK1 (phospho T212)
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PAK1 (phospho T212) antibody
See all PAK1 primary antibodies -
Description
Rabbit polyclonal to PAK1 (phospho T212) -
Host species
Rabbit -
Specificity
ab75599 detects endogenous levels of PAK1 only when phosphorylated at threonine 212. -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic phosphopeptide derived from human PAK1 around the phosphorylation site of threonine 212 (P-V-TP-P-T).
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Positive control
- Extracts from 293 cells treated with forskolin (40µM, 30min). Human breast carcinoma tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab75599 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against the non-phosphopeptide was removed by chromatography using a non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PAK1 (phospho T212) antibody (ab75599)All lanes : Anti-PAK1 (phospho T212) antibody (ab75599) at 1/500 dilution
Lane 1 : Extracts from 293 cells (untreated)
Lane 2 : Extracts from 293 cells treated with forskolin (40µM, 30min),
Predicted band size: 61 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK1 (phospho T212) antibody (ab75599)Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab75599, at 1/50 dilution, in the absence (left panel) and presence (right panel) of the immunising phosphopeptide. -
Immunocytochemistry/ Immunofluorescence - Anti-PAK1 (phospho T212) antibody (ab75599)ab75599 at 1/100 dilution staining PAK1 (phospho T212) in Hela cells by ICC/IF (MeOH fixation).
