HRP Anti-ERK2 antibody [E460] (ab205819)
![HRP Anti-ERK2 antibody [E460] (ab205819)](https://www.abcam.com/ps/products/205/ab205819/Images/ab205819-261166-anti-erk2-antibody-e460-hrp-western-blot.jpg "HRP Anti-ERK2 antibody [E460] (ab205819)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [E460] to ERK2
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human, Recombinant fragment
- Conjugation: HRP
Overview
-
Product name
HRP Anti-ERK2 antibody [E460]
See all ERK2 primary antibodies -
Description
HRP Rabbit monoclonal [E460] to ERK2 -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHumanRecombinant fragment
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: HeLa, 46C, PC12 whole cell lysates and Human ERK2 recombinant protein lysate.
-
General notes
Alternative versions available:
Anti-ERK2 antibody [E460] (ab32081) - Knockout validatedThis product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
E460 -
Isotype
IgG -
Research areas
Images
-
Western blot - HRP Anti-ERK2 antibody [E460] (ab205819)All lanes : HRP Anti-ERK2 antibody [E460] (ab205819) at 1/5000 dilution
Lane 1 :HeLa whole cell lysate (ab150035) at 10 µg
Lane 2 : 46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate at 10 µg
Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg
Lane 4 : Recombinant Human ERK1 protein (ab43623) (ab43623) at 0.1 µg
Lane 5 :Recombinant Human ERK2 protein (ab43625) at 0.1 µg
Lane 6 : Wild-type HAP1 cell lysate at 20 µg
Lane 7 : ERK2 knockout HAP1 cell lysate at 20 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 41 kDa
Observed band size: 41 kDa
Exposure time: 12 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab205819 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
-
![HRP Anti-ERK2 antibody [E460] (ab205819)](https://www.abcam.com/ps/products/205/ab205819/Images/ab205819-3-benefits-of-recombinant-antibodies.png)
HRP Anti-ERK2 antibody [E460] (ab205819)
