Anti-ERK2 antibody (ab227089)
Key features and details
- Rabbit polyclonal to ERK2
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ERK2 antibody
See all ERK2 primary antibodies -
Description
Rabbit polyclonal to ERK2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human ERK2 (N terminal). The exact sequence is proprietary. (Carrier-protein conjugated).
Database link: P28482 -
Positive control
- WB: HeLa cell extract; A431, H1299 and HepG2 whole cell lysates. IHC-P: Human oral carcinoma tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 59.99% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ERK2 antibody (ab227089)All lanes : Anti-ERK2 antibody (ab227089) at 1/500 dilution
Lane 1 : WT HeLa cell extract
Lane 2 : ERK2 Knockout HeLa cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 41 kDa
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Western blot - Anti-ERK2 antibody (ab227089)All lanes : Anti-ERK2 antibody (ab227089) at 1/500 dilution
Lane 1 : Wild-type HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 2 : ERK2 knockout HeLa cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 41 kDa10% SDS-PAGE gel.
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Western blot - Anti-ERK2 antibody (ab227089)All lanes : Anti-ERK2 antibody (ab227089) at 1/1500 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : H1299 whole cell lysate
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 41 kDa12% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK2 antibody (ab227089)Paraffin-embedded human oral carcinoma tissue stained for ERK2 with ab227089 at 1/500 dilution in immunohistochemical analysis.
