Anti-SOD2/MnSOD antibody (ab13533)
Key features and details
- Rabbit polyclonal to SOD2/MnSOD
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-SOD2/MnSOD antibody
See all SOD2/MnSOD primary antibodies -
Description
Rabbit polyclonal to SOD2/MnSOD -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRat
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Immunogen
Full length protein corresponding to Human SOD2/MnSOD.
Database link: P04179
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was immunoaffinity purified. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SOD2/MnSOD antibody (ab13533)All lanes : Anti-SOD2/MnSOD antibody (ab13533) at 1/1000 dilution (o/n at 4degC)
Lane 1 : Mouse brain
Lane 2 : Rat brain
Lane 3 : Rat brain membrane
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP for 1 hr at RT with shaking at 1/5000 dilution
Predicted band size: 26.6 kDaBlock: 5% Skim Milk powder in TBST.
Color Development: Chemiluminescent for HRP for 5 min at RT.
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Western blot - Anti-SOD2/MnSOD antibody (ab13533) Image courtesy of anonymous abreviewerAll lanes : Anti-SOD2/MnSOD antibody (ab13533) at 1/5000 dilution
Lane 1 : mouse liver whole cell lysate
Lane 2 : mouse hepatocytes whole cell lysate
Secondary
All lanes : Goat anti-rabbit HRP conjugate at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 26.6 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?
Exposure time: 15 seconds
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Western blot - Anti-SOD2/MnSOD antibody (ab13533) This image is courtesy of an Abreview submitted by Dr sandra sobocanecAll lanes : Anti-SOD2/MnSOD antibody (ab13533) at 1/5000 dilution
Lane 1 : 2ug mouse liver homogenate supernatant
Lane 2 : 1ug mouse liver homogenate supernatant
Lane 3 : 0.5ug mouse liver homogenate supernatant
Secondary
All lanes : HRP conjugated donkey anti-rabbit antibody
Performed under reducing conditions.
Predicted band size: 26.6 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds
