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Anti-SOD2/MnSOD (acetyl K122) antibody [NCI-R156-33] - BSA and Azide free (ab240391)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [NCI-R156-33] to SOD2/MnSOD (acetyl K122) - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Human

Overview

  • Product name

    Anti-SOD2/MnSOD (acetyl K122) antibody [NCI-R156-33] - BSA and Azide free
    See all SOD2/MnSOD primary antibodies

  • Description

    Rabbit monoclonal [NCI-R156-33] to SOD2/MnSOD (acetyl K122) - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab240391 is the carrier-free version of ab214675. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240391 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Western blot - Anti-SOD2/MnSOD (acetyl K122) antibody [NCI-R156-33] - BSA and Azide free (ab240391)
    Western blot - Anti-SOD2/MnSOD (acetyl K122) antibody [NCI-R156-33] - BSA and Azide free (ab240391)

    This data was developed using ab214675, the same antibody clone in a different buffer formulation.

    Flag-SOD2/MnSOD (acetyl K122) was immunoprecipitated from HEK293T cells, transfected with Flag-tagged SOD2/MnSOD (acetyl K122) expression vector and treated with 1µM Trichostatin A (TSA) for 48 h, with anti-Flag antibody.

    Western blot was performed from the immunoprecipitate using ab214675 at 1/200 dilution.

    Horseradish peroxidase (HRP)-conjugated anti-Rabbit IgG, was used as secondary antibody at 1/10000 dilution.

    Lane 1: IP samples washed and incubated with purified SIRT3 protein without NAD+, 10 µg.
    Lane 2: IP samples washed and incubated with purified SIRT3 protein with NAD+ for two hours, 10 µg.
    Lane 3: IP samples washed and incubated with purified SIRT3 protein without NAD+, then blocked with acetylated peptide (Ac-peptide), 10 µg.
    Lane 4: IP samples washed and incubated with purified SIRT3 protein with NAD+ for two hours, then blocked with acetylated peptide (Ac-peptide), 10 µg.
    Lane 5: IP samples washed and incubated with purified SIRT3 protein without NAD+, then blocked with non-acetylated control peptide (cont-peptide), 10 µg.
    Lane 6: IP samples washed and incubated with purified SIRT3 protein with NAD+ for two hours, then blocked with non-acetylated control peptide (cont-peptide), 10 µg.

    SIRT3 with NAD+  decreased SOD2/MnSOD acetylation in vitro. The expression profile observed is consistent with what has been described in the literature (PMID: 21172655; 25852572). This data was kindly provided by Dr. Gius (Northwestern University), and has been published in PMID: 21172655.

  • Anti-SOD2/MnSOD (acetyl K122) antibody [NCI-R156-33] - BSA and Azide free (ab240391)
    Anti-SOD2/MnSOD (acetyl K122) antibody [NCI-R156-33] - BSA and Azide free (ab240391)

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