Anti-SOD2/MnSOD antibody (ab13534)
Key features and details
- Rabbit polyclonal to SOD2/MnSOD
- Suitable for: ICC/IF, IHC-P, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-SOD2/MnSOD antibody
See all SOD2/MnSOD primary antibodies -
Description
Rabbit polyclonal to SOD2/MnSOD -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P MouseIP MouseRat
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Immunogen
Full length protein corresponding to Rat SOD2/MnSOD.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was immunoaffinity purified. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD antibody (ab13534)
ab13534 at 1/100 dilution staining Superoxide Dismutase 2 in mouse back skin tissue section by Immunohistochemistry (Bouin's fixative fixed paraffin-embedded tissue section). Antigen retrieval was done by microwave in citrate buffer. A Fluorophore-conjugated goat anti-rabbit secondary was used at 1/50 dilution.
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Immunocytochemistry/ Immunofluorescence - Anti-SOD2/MnSOD antibody (ab13534)Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling SOD2/MnSOD with ab13534. Cells were fixed with 2% formaldehyde for 20 minutes at room temperature and incubated with the primary antibody at a dilution of 1/120 for 12 hours at 4°C. Followed by incubation with a R-PE-conjugated goat anti-rabbit IgG at a dilution of 1/200 for 2 hours at room temperature. Counterstained with DAPI (blue) at 1/40000 for 2 hours at room temperature. Magnification: 100x.
Left: DAPI (blue) nuclear stain. Middle: SOD2/MnSOD. Right: Merge.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD antibody (ab13534) This image is courtesy of an anonymous Abreviewab13534 staining SOD2 in Human colon and Horse Ileo tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/2000) for 18 hours.
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Immunocytochemistry/ Immunofluorescence - Anti-SOD2/MnSOD antibody (ab13534) This image is courtesy of an Abreview submitted by Dr Anne Chiaramelloab13534 staining differentiating (rat) PC12 cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.2% Triton X100 prior to blocking in 10% serum at RT for 1 hour. The primary antibody was incubated with the sample for 1 hour at 2 µg/ml. An Alexa Fluor® 488 conjugated goat anti-rabbit antibody diluted 1/1000 was used as the secondary. Cells were counterstained with the nuclear TOPRO-3 stain.
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Immunoprecipitation - Anti-SOD2/MnSOD antibody (ab13534)Lane 1: ab13534 + Mouse brain lysate (250µg)
Lane 2: ab13534 + Mouse brain lysate (250µg, ½ dilution)
Lane 3: Mouse brain lysate (15µg)
Lane 4: Rat brain lysate (15µg)Ab13534 at 1:1000 immunoprecipitating SOD2 in mouse brain and rat brain lysate. For western blotting, donkey anti- rabbit IgG (HRP) was used as the secondary antibody.
