Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)
![Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)](https://www.abcam.com/ps/products/68/ab68155/Images/ab68155-5-anti-sod2-antibody-epr2560y-western-blot-human-lysate.jpg "Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2560Y] to SOD2/MnSOD
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-SOD2/MnSOD antibody [EPR2560Y]
See all SOD2/MnSOD primary antibodies -
Description
Rabbit monoclonal [EPR2560Y] to SOD2/MnSOD -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB RatHuman
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Immunogen
Synthetic peptide within Human SOD2/MnSOD aa 1-100 (N terminal). The exact sequence is proprietary.
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Positive control
- WB: SH-SY5Y, A549, HEK-293T and HAP1 cell lysates; Human heart tissue; Rat brain and heart lysate. IHC-P: Human liver tissue sections. IHC-Fr: Rat lung tissue sections.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR2560Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)All lanes : Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155) at 1/1000 dilution
Lane 1 : SHSY5Y cell lysate
Lane 2 : A549 cell lysate
Lane 3 : Wild-type HEK-293T cell lysate
Lane 4 : SOD2 knockout HEK-293T cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 25 kDaLanes 1 - 4: Merged signal (red and green). Green - ab68155 observed at 22 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab68155 was shown to react with SOD2/MnSOD in wild-type HEK-293T. Loss of signal was observed when knockout cell line ab255434 (knockout cell lysate ab263835) was used. Wild-type and SOD2/MnSOD knockout samples were subjected to SDS-PAGE. ab68155 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)](https://www.abcam.com/ps/products/68/ab68155/Images/ab68155-261350-ab68155SOD2.jpg)
Western blot - Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)All lanes : Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155) at 1/1000 dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : SOD2 knockout HAP1 cell lysate
Lane 3 : Human heart tissue lysate
Lane 4 : Rat heart tissue lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 25 kDaLanes 1 - 4: Merged signal (red and green). Green - ab68155 observed at 29 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab68155 was shown to specifically react with SOD2 when SOD2 knockout samples were used. Wild-type and SOD2 knockout samples were subjected to SDS-PAGE. ab68155 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging. -
![Western blot - Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)](https://www.abcam.com/ps/products/68/ab68155/Images/ab68155_1.jpg)
Western blot - Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)All lanes : Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155) at 1/500 dilution
Lane 1 : Rat brain lysate
Lane 2 : SH-SY5Y lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)](https://www.abcam.com/ps/products/68/ab68155/Images/ab68155_3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)ab68155 at 1:100 dilution staining Superoxide Dismutase 2 in human liver tissue sections.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)](https://www.abcam.com/ps/products/68/ab68155/Images/ab68155-6-benefits-of-recombinant-antibodies.png)
Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)
