Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SOD2/MnSOD antibody [EPR2560Y] - BSA and Azide free (ab227846)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2560Y] to SOD2/MnSOD - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SOD2/MnSOD antibody [EPR2560Y] - BSA and Azide free
    See all SOD2/MnSOD primary antibodies

  • Description

    Rabbit monoclonal [EPR2560Y] to SOD2/MnSOD - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Rat brain lysate, SH-SY5Y, A549 and HEK-293T cell lysates. IHC-P: Human liver tissue sections.

  • General notes

    Ab227846 is the carrier-free version of ab68155. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab227846 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Images

  • Western blot - Anti-SOD2/MnSOD antibody [EPR2560Y] - BSA and Azide free (ab227846)
    Western blot - Anti-SOD2/MnSOD antibody [EPR2560Y] - BSA and Azide free (ab227846)
    All lanes : Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155) at 1/1000 dilution

    Lane 1 : SHSY5Y cell lysate
    Lane 2 : A549 cell lysate
    Lane 3 : Wild-type HEK-293T cell lysate
    Lane 4 : SOD2 knockout HEK-293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 25 kDa
    Observed band size: 29 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab68155).

    Lanes 1 - 4: Merged signal (red and green). Green - ab68155 observed at 22 kDa. Red - loading control, ab8245 observed at 37 kDa.  

     ab68155 was shown to react with SOD2/MnSOD in wild-type HEK-293T. Loss of signal was observed when knockout cell line ab255434 (knockout cell lysate ab263835) was used. Wild-type and SOD2/MnSOD knockout samples were subjected to SDS-PAGE. ab68155 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

     

  • Western blot - Anti-SOD2/MnSOD antibody [EPR2560Y] - BSA and Azide free (ab227846)
    Western blot - Anti-SOD2/MnSOD antibody [EPR2560Y] - BSA and Azide free (ab227846)
    All lanes : Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 cell lysate
    Lane 2 : SOD2 knockout HAP1 cell lysate
    Lane 3 : Human heart tissue lysate
    Lane 4 : Rat heart tissue lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 25 kDa



    This WB data was generated using the same anti-SOD2 antibody clone, EPR2560Y, in a different buffer formulation (Cat# ab68155).


    Lanes 1 - 4: Merged signal (red and green). Green - ab68155 observed at 29 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab68155 was shown to specifically react with SOD2 when SOD2 knockout samples were used. Wild-type and SOD2 knockout samples were subjected to SDS-PAGE. ab68155 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD antibody [EPR2560Y] - BSA and Azide free (ab227846)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD antibody [EPR2560Y] - BSA and Azide free (ab227846)

    This IHC data was generated using the same anti-SOD2 antibody clone, EPR2560Y, in a different buffer formulation (cat# ab68155).

    ab68155 at 1:100 dilution staining Superoxide Dismutase 2 in human liver tissue sections.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-SOD2/MnSOD antibody [EPR2560Y] - BSA and Azide free (ab227846)
    Anti-SOD2/MnSOD antibody [EPR2560Y] - BSA and Azide free (ab227846)

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