HRP Anti-SDHB antibody [21A11AE7] (ab197903)
![HRP Anti-SDHB antibody [21A11AE7] (ab197903)](https://www.abcam.com/ps/products/197/ab197903/Images/ab197903-355093-anti-sdhb-antibody-21a11ae7-western-blot-knock-out-hek293-sdhb-hepg2-human.jpg "HRP Anti-SDHB antibody [21A11AE7] (ab197903)")
Key features and details
- HRP Mouse monoclonal [21A11AE7] to SDHB
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
- Conjugation: HRP
- Isotype: IgG2a
Overview
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Product name
HRP Anti-SDHB antibody [21A11AE7]
See all SDHB primary antibodies -
Description
HRP Mouse monoclonal [21A11AE7] to SDHB -
Host species
Mouse -
Conjugation
HRP -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Zebrafish
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Immunogen
Tissue, cells or virus corresponding to Cow SDHB. Purified Bovine mitochondrial Complex II, with traces of Complex III.
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Positive control
- WB: Human heart mitochondria lysate. IHC-P: FFPE normal human colon tissue sections.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
21A11AE7 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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Western blot - HRP Anti-SDHB antibody [21A11AE7] (ab197903)All lanes : HRP Anti-SDHB antibody [21A11AE7] (ab197903) at 1/5000 dilution
Lane 1 : Wild-type HEK-293 whole cell lysate
Lane 2 : SDHB knockout HEK-293 whole cell lysate
Lane 3 : Hep G2 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 32 kDa
Observed band size: 32 kDaLanes 1 - 3: Merged signal (red and green). Green - ab197903 observed at 32 kDa. Red - loading control, ab130007, observed at 130 kDa.
ab197903 was shown to specifically react with SDHB in wild-type HEK-293 cells as signal was lost in SDHB knockout cells. Wild-type and SDHB knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab197903 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-SDHB antibody [21A11AE7] (ab197903)](https://www.abcam.com/ps/products/197/ab197903/Images/ab197903-255253-IHCAP22401951.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-SDHB antibody [21A11AE7] (ab197903)IHC image of SDHB staining in a section of formalin-fixed paraffin-embedded human normal colon*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab197903 at 1/100 dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![Western blot - HRP Anti-SDHB antibody [21A11AE7] (ab197903)](https://www.abcam.com/ps/products/197/ab197903/Images/ab197903-238355-WBab1979031.jpg)
Western blot - HRP Anti-SDHB antibody [21A11AE7] (ab197903)HRP Anti-SDHB antibody [21A11AE7] (ab197903) at 1/5000 dilution + Human heart tissue lysate - mitochondrial extract (ab110337) at 5 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 32 kDa
Observed band size: 32 kDa
Exposure time: 6 secondsThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab197903 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
