Alexa Fluor® 488 Anti-SDHB antibody [21A11AE7] (ab197902)
![Alexa Fluor® 488 Anti-SDHB antibody [21A11AE7] (ab197902)](https://www.abcam.com/ps/products/197/ab197902/Images/ab197902-396951-antiSDHBantibodyimmunocytochemistryimmunofluorescencehek293human.jpg "Alexa Fluor® 488 Anti-SDHB antibody [21A11AE7] (ab197902)")
Key features and details
- Alexa Fluor® 488 Mouse monoclonal [21A11AE7] to SDHB
- Suitable for: ICC/IF
- Knockout validated
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
- Isotype: IgG2a
Overview
-
Product name
Alexa Fluor® 488 Anti-SDHB antibody [21A11AE7]
See all SDHB primary antibodies -
Description
Alexa Fluor® 488 Mouse monoclonal [21A11AE7] to SDHB -
Host species
Mouse -
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Human
-
Immunogen
Tissue, cells or virus corresponding to Cow SDHB. Purified Bovine mitochondrial Complex II, with traces of Complex III.
-
Positive control
- ICC/IF: HeLa cells. HEK293 (HEK293-SDHB KO used as negative cells).
-
General notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
21A11AE7 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
-
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SDHB antibody [21A11AE7] (ab197902)
ab197902 staining SDHB in wild-type HEK293 cells (top panel) and SDHB knockout HEK293 cells (bottom panel). The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab197902 at 1/500 dilution (shown in green) and ab190573 (Rabbit monoclonal to alpha Tubulin - Alexa Fluor® 647) at 1/250 dilution (shown in red) overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). -
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SDHB antibody [21A11AE7] (ab197902)](https://www.abcam.com/ps/products/197/ab197902/Images/ab197902-239482-ICCAP223740601.jpg)
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SDHB antibody [21A11AE7] (ab197902)ab197902 staining SDHB in HeLa cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab197902 at 1/250 dilution (shown in green) and ab6046 (Rabbit polyclonal to beta Tubulin) at 1µg/ml. This was followed by an incubation at room temperature for 1h with ab150088, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594), pre-adsorbed, at 1µg/ml (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
