Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸ 10 µl 127 315 ₸

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Anti-SDHB antibody [EPR10880] (ab175225)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10880] to SDHB
Suitable for: WB, IP, Flow Cyt, IHC-P
Knockout validated
Reacts with: Mouse, Rat, Human

Product name

Anti-SDHB antibody [EPR10880]
See all SDHB primary antibodies
Description

Rabbit monoclonal [EPR10880] to SDHB
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Mouse Rat Human
IP	Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide within Human SDHB aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: P21912
Positive control
- WB: HepG2, A431 and Jurkat whole cell lysate (ab7899) and human fetal heart, mouse brain and rat brain tissue lysates. IHC-P: Human caratoid paraganglioma, human mesenchymoma, human kidney, human liver, mouse kidney and rat kidney tissues. Flow Cyt: Jurkat and A431 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR10880
Isotype

IgG
Research areas

Western blot - Anti-SDHB antibody [EPR10880] (ab175225)

All lanes : Anti-SDHB antibody [EPR10880] (ab175225) at 1/50000 dilution

Lane 1 : Wild-type HEK 293 whole cell lysate
Lane 2 : SDHB knockout HEK 293 whole cell lysate
Lane 3 : HepG2 whole cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 32 kDa

Lanes 1 - 3: Merged signal (red and green). Green - ab175225 observed at 32 kDa. Red - loading control, ab130007, observed at 130 kDa.

ab175225 was shown to recognize SDHB in wild-type HEK 293 cells as signal was lost at the expected MW in SDHB knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and SDHB knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab175225 and ab130007 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/50000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-SDHB antibody [EPR10880] (ab175225)

All lanes : Anti-SDHB antibody [EPR10880] (ab175225) at 1/100000 dilution (purified)

Lane 1 : HepG2 whole cell lysate
Lane 2 : Jurkat whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 32 kDa
Observed band size: 32 kDa

Blocking and dilution buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHB antibody [EPR10880] (ab175225)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling SDHB with purified ab175225 at a dilution of 1/2000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHB antibody [EPR10880] (ab175225)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labeling SDHB with unpurified ab175225 at a dilution of 1/100.
Western blot - Anti-SDHB antibody [EPR10880] (ab175225)

All lanes : Anti-SDHB antibody [EPR10880] (ab175225) at 1/100000 dilution (purified)

Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lane 3 : Mouse spleen tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 32 kDa
Observed band size: 32 kDa

Blocking and dilution buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHB antibody [EPR10880] (ab175225)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human mesenchymoma tissue labelling SDHB with purified ab175225 at a dilution of 1/2000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHB antibody [EPR10880] (ab175225)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue labelling SDHB with purified ab175225 at a dilution of 1/2000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHB antibody [EPR10880] (ab175225)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue labelling SDHB with purified ab175225 at a dilution of 1/2000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Flow Cytometry - Anti-SDHB antibody [EPR10880] (ab175225)

Flow Cytometry analysis of A431 cells labelling SDHB with purified ab175225 at a dilution of 1/200 (red). Cells were fixed with 4% paraformaldehyde. An Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
Immunoprecipitation - Anti-SDHB antibody [EPR10880] (ab175225)

ab175225 (purified) at a dilution of 1/60 immunoprecipitating SDHB in Jurkat whole cell lysate.

Lane 1 (input): Jurkat whole cell lysate (10µg)

Lane 2 (+): ab175225 + Jurkat whole cell lysate.

Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab175225 in Jurkat whole cell lysate.

For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Blocking buffer and concentration: 5% NFDM/TBST.

Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-SDHB antibody [EPR10880] (ab175225)

All lanes : Anti-SDHB antibody [EPR10880] (ab175225) at 1/50000 dilution (unpurified)

Lane 1 : HepG2 cell lysate
Lane 2 : A431 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Fetal heart tissue lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 32 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHB antibody [EPR10880] (ab175225)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labeling SDHB with unpurified ab175225 at a dilution of 1/100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHB antibody [EPR10880] (ab175225)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human carotid paraganglioma tissue labeling SDHB with unpurified ab175225 at a dilution of 1/100.
Flow Cytometry - Anti-SDHB antibody [EPR10880] (ab175225)

Flow cytometric analysis of permeabilized Jurkat cells labeling SDHB with unpurified ab175225 at a dilution of 1/10 (red) compared to a rabbit IgG negative control (green).
Anti-SDHB antibody [EPR10880] (ab175225)

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