Anti-Granzyme B antibody [EPR22645-244] - BSA and Azide free (ab255869)
![Anti-Granzyme B antibody [EPR22645-244] - BSA and Azide free (ab255869)](https://www.abcam.com/ps/products/255/ab255869/Images/ab255869-1-anti-granzyme-b-antibody-epr22645-244-flowcytometry-spleen-mouse.jpg "Anti-Granzyme B antibody [EPR22645-244] - BSA and Azide free (ab255869)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22645-244] to Granzyme B - BSA and Azide free
- Suitable for: Flow Cyt, WB
- Reacts with: Mouse
Overview
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Product name
Anti-Granzyme B antibody [EPR22645-244] - BSA and Azide free
See all Granzyme B primary antibodies -
Description
Rabbit monoclonal [EPR22645-244] to Granzyme B - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Mouse
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse splenocyte lysate Flow cyt: Mouse splenocyte cells treated with 2.5ug/ml Concanavalin A for 72h.
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General notes
Ab255869 is the carrier-free version of ab255599. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab255869 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22645-244 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-Granzyme B antibody [EPR22645-244] - BSA and Azide free (ab255869)
Flow cytometric analysis of 2% paraformaldehyde-fixed, 0.1% Tween-20 permeabilized mouse splenocyte (treated with 2.5ug/ml Concanavalin A for 72h) cells labeling Granzyme B with ab255599 at 1/800 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left).
Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Cells were surface stained with anti-CD8 alpha conjugated to Alexa Fluor® 647.Then fixed with 2% PFA for 10min and intracellularly stained with rabbit IgG (Left) or ab255599 (Right).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255599).
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![Anti-Granzyme B antibody [EPR22645-244] - BSA and Azide free (ab255869)](https://www.abcam.com/ps/products/255/ab255869/Images/ab255869-2-benefits-of-recombinant-antibodies.png)
Anti-Granzyme B antibody [EPR22645-244] - BSA and Azide free (ab255869)
