Anti-NUP214 antibody (ab264143)
Key features and details
- Rabbit polyclonal to NUP214
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-NUP214 antibody
See all NUP214 primary antibodies -
Description
Rabbit polyclonal to NUP214 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB MouseHuman
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Immunogen
Synthetic peptide within Human NUP214 aa 2050-2090 (C terminal). The exact sequence is proprietary.
Database link: P35658 -
Positive control
- WB: HeLa, HEK-293T and NIH/3T3 whole cell lysate. IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NUP214 antibody (ab264143)All lanes : Anti-NUP214 antibody (ab264143) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T whole cell lysate at 50 µg
Lane 5 : NIH/3T3 whole cell lysate at 50 µg
Exposure time: 3 minutes4-8% SDS-PAGE
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Immunoprecipitation - Anti-NUP214 antibody (ab264143)NUP214 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264143 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264143 at 1 µg/ml.
Lane 1: ab264143 IP in HeLa whole cell lysate.
Lane 2: Control IgG.Exposure time: 30 secs.
