Antibodies, Proteins, Kits and Reagents for Life Science

335 040 ₸ Product size

100 µl 335 040 ₸ 10 µl 137 366 ₸

Order now and get it on Tuesday March 02, 2021

Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPVANR2] to SOD2/MnSOD (acetyl K68)
Suitable for: IHC-P, WB, Dot blot
Reacts with: Mouse, Rat, Human

Product name

Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2]
See all SOD2/MnSOD primary antibodies
Description

Rabbit monoclonal [EPVANR2] to SOD2/MnSOD (acetyl K68)
Host species

Rabbit
Specificity

ab137037 only detects SOD2/MnSOD when acetylated at Lysine 68. According to BLAST analysis, the antibody might cross-react with Fer (Uniprot P70451) isoform 3 in mouse samples. No experiment has been done to confirm this possibility.

Tested Applications & Species

Application	Species
IHC-P	Mouse Rat Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide within Human SOD2/MnSOD (acetyl K68). The exact sequence is proprietary.
Database link: P04179
(Peptide available as ab176149)
Positive control
- WB: Trichostatin A treated SOD2 transfected HEK-293T lysates with non-acetyl peptide. Trichostatin A treated SOD2 transfected HEK-293T lysates. Mouse heart lysate. Rat kidney tissue lysate. SOD2 transfected HEK-293T lysate. IHC-P: Human cervical carcinoma. Human carcinoma kidney tissue. Rat liver tissue. Mouse kidney tissue. Human muscle tissue.
General notes
This antibody was developed as part of a collaboration with the lab of David Guis at Vanderbilt University.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPVANR2
Isotype

IgG
Research areas

Western blot - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)

All lanes : Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037) at 1/10000 dilution (purified)

Lane 1 : Trichostatin A treated SOD2 transfected HEK-293T lysates with acetyl peptide
Lane 2 : Trichostatin A treated SOD2 transfected HEK-293T lysates with non-acetyl peptide
Lane 3 : Trichostatin A treated SOD2 transfected HEK-293T lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 24 kDa

Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human clear cell carcinoma kidney tissue labelling SOD2 (acetyl K68) with purified ab137037 at 1/150. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody (1/500).

Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Western blot - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)

Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037) at 1/1000 dilution (purified) + Mouse heart lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

Predicted band size: 24 kDa

Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue labelling SOD2 (acetyl K68) with purified ab137037 at 1/150. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody (1/500).

Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Western blot - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)

Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037) at 1/1000 dilution (purified) + Rat kidney tissue lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

Predicted band size: 24 kDa

Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue labelling SOD2 (acetyl K68) with purified ab137037 at 1/150. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody (1/500).

Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical carcinoma tissue labelling SOD2 (acetyl K68) with unpurified ab137037 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)

All lanes : Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037) at 1/1000 dilution (unpurified)

Lane 1 : Non transfected HEK-293T lysate
Lane 2 : SOD2 transfected HEK-293T lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP conjugated Goat anti Rabbit IgG at 1/2000 dilution

Predicted band size: 24 kDa
Western blot - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)

All lanes : Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037) at 1/1000 dilution (unpurified)

Lane 1 : Non transfected HEK-293T cell lysate with with Acetyl SOD2 (K68) peptide
Lane 2 : SOD2 transfected HEK-293T cell lysate with control peptide

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP conjugated Goat anti Rabbit IgG at 1/2000 dilution

Predicted band size: 24 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labelling SOD2 (acetyl K68) with unpurified ab137037 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Dot Blot - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)

Dot blot analysis labelling SOD2 (acetyl K68) with ab137037 at a dilution of 1/1000. Peroxidase conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.

Blocking and diluting buffer: 5% NFDM/TBST.

Lane 1: SOD2 (acetyl K68) acetylated peptide.

Lane 2: SOD2 Non-acetylated peptide.

Exposure time: 3 minutes.
Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)

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