Anti-NDUFA9 antibody (ab128744)
Key features and details
- Rabbit polyclonal to NDUFA9
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-NDUFA9 antibody
See all NDUFA9 primary antibodies -
Description
Rabbit polyclonal to NDUFA9 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
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Positive control
- This antibody gave a positive signal in both Mouse Testis and Heart tissue lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab128744 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseAll applications RabbitHorseDogHumanChimpanzeeGorillaChinese hamsterOrangutanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 42 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 42 kDa).Target
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Function
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. -
Sequence similarities
Belongs to the complex I NDUFA9 subunit family. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 4704 Human
- Entrez Gene: 66108 Mouse
- Omim: 603834 Human
- SwissProt: Q16795 Human
- SwissProt: Q9DC69 Mouse
- Unigene: 75227 Human
- Unigene: 29939 Mouse
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Alternative names
- CI-39kD antibody
- Complex I subunit NDUFA9 antibody
- Complex I-39kD antibody
see all
Images
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Western blot - Anti-NDUFA9 antibody (ab128744)All lanes : Anti-NDUFA9 antibody (ab128744) at 1 µg/ml
Lane 1 : Testis (Mouse) Tissue Lysate
Lane 2 : Heart (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa, 58 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
The band observed at 35 kDa could potentially be a cleaved form of NDUFA9 due to the presence of a 35 amino acid transit peptide. This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab128744 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Datasheets and documents
References (3)
ab128744 has been referenced in 3 publications.
- Zhang F et al. P53 and Parkin co-regulate mitophagy in bone marrow mesenchymal stem cells to promote the repair of early steroid-induced osteonecrosis of the femoral head. Cell Death Dis 11:42 (2020). PubMed: 31959744
- Li K et al. Maternally Inherited Diabetes Mellitus Associated with a Novel m.15897G>A Mutation in Mitochondrial tRNAThr Gene. J Diabetes Res 2020:2057187 (2020). PubMed: 32083134
- Liu T et al. Mechanisms of ROS-induced mitochondria-dependent apoptosis underlying liquid storage of goat spermatozoa. Aging (Albany NY) 11:7880-7898 (2019). PubMed: 31548434
Images
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Western blot - Anti-NDUFA9 antibody (ab128744)All lanes : Anti-NDUFA9 antibody (ab128744) at 1 µg/ml
Lane 1 : Testis (Mouse) Tissue Lysate
Lane 2 : Heart (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa, 58 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
The band observed at 35 kDa could potentially be a cleaved form of NDUFA9 due to the presence of a 35 amino acid transit peptide. This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab128744 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
