HRP Anti-NNT antibody [8B4BB10] (ab198312)
![HRP Anti-NNT antibody [8B4BB10] (ab198312)](https://www.abcam.com/ps/products/198/ab198312/Images/ab198312-244676-anti-nnt-antibody-8b4bb10-hrp-western-blot.jpg "HRP Anti-NNT antibody [8B4BB10] (ab198312)")
Key features and details
- HRP Mouse monoclonal [8B4BB10] to NNT
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
- Isotype: IgG1
Overview
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Product name
HRP Anti-NNT antibody [8B4BB10]
See all NNT primary antibodies -
Description
HRP Mouse monoclonal [8B4BB10] to NNT -
Host species
Mouse -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Tissue, cells or virus corresponding to Human NNT.
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Positive control
- WB: HepG2 whole cell lysate.
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General notes
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Proprietary Purification -
Purification notes
Produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Purity >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
8B4BB10 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - HRP Anti-NNT antibody [8B4BB10] (ab198312)HRP Anti-NNT antibody [8B4BB10] (ab198312) at 1/5000 dilution + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 114 kDa
Observed band size: 114 kDa
Exposure time: 8 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab198312 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
