Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] (ab126606)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7163] to Sumo 2 + Sumo 3 + Sumo 4
  • Suitable for: WB, Flow Cyt, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163]
    See all Sumo 2 + Sumo 3 + Sumo 4 primary antibodies

  • Description

    Rabbit monoclonal [EPR7163] to Sumo 2 + Sumo 3 + Sumo 4

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Sumo 2 + Sumo 3 + Sumo 4 aa 50-150 (Cysteine residue). The exact sequence is proprietary. The immunogen sequence is based upon a region in Sumo 4 that has significant homology with Sumo 2 and Sumo 3.
    Database link: Q6EEV6

  • Positive control

    • HepG3, 293T and Jurkat whole cell lysate (ab7899); Human spleen tissue lysate; HepG2 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] (ab126606)
    Flow Cytometry - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] (ab126606)
    Overlay histogram showing HEK293 cells stained with ab126606 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab126606, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Immunocytochemistry/ Immunofluorescence - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] (ab126606)
    Immunocytochemistry/ Immunofluorescence - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] (ab126606)

    ab126606 at 1/250 dilution staining Sumo 2 + Sumo 3 + Sumo 4 in HepG2 (Human liver hepatocellular carcinoma cell line) cells by immunofluorescence.

  • Western blot - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] (ab126606)
    Western blot - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] (ab126606)
    All lanes : Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] (ab126606) at 1/10000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : Human spleen tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 11 kDa

  • Western blot - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] (ab126606)
    Western blot - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] (ab126606)
    All lanes : Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] (ab126606) at 1/5000 dilution

    Lane 1 : Recombinant Human Sumo 1 protein (ab140417) at 0.01 µg
    Lane 2 : Recombinant Human Sumo 2 protein (ab140420) at 0.01 µg
    Lane 3 : Recombinant Human Sumo 3 protein (ab140414) at 0.01 µg
    Lane 4 : Recombinant Human Sumo 4 protein (ab157025) at 0.1 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 11 kDa
    Observed band size: 16 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 seconds


    Blocking buffer: 5% NFDM/TBST
  • OI-RD Scanning - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] (ab126606)
    OI-RD Scanning - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] (ab126606)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] (ab126606)
    Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] (ab126606)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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