Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14880] to SAE2 / UBA2
  • Suitable for: IHC-P, IP, ICC/IF, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SAE2 / UBA2 antibody [EPR14880]
    See all SAE2 / UBA2 primary antibodies

  • Description

    Rabbit monoclonal [EPR14880] to SAE2 / UBA2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Mouse
    Human
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, A549, K562, Raw264.7 and PC-12 cell lysates; Human colon tissue; Mouse brain tissue; HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR14880

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955)
    Western blot - Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955)
    All lanes : Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : A549 cell lysate
    Lane 3 : K562 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 71 kDa
    Observed band size: 90 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955)
    Western blot - Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955)
    All lanes : Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955) at 1/1000 dilution

    Lane 1 : Raw264.7 cell lysate
    Lane 2 : PC-12 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 71 kDa
    Observed band size: 90 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SAE2 / UBA2 with ab185955 at 1/50 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955)

    Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling SAE2 / UBA2 with ab185955 at 1/50 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955)
    Immunocytochemistry/ Immunofluorescence - Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955)

    Immunofluorescent analysis of acetone-fixed HeLa cells labeling SAE2 / UBA2 with ab185955 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution.

  • Immunoprecipitation - Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955)
    Immunoprecipitation - Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955)

    Western blot analysis of SAE2 / UBA2 in K562 cell lysate immunoprecipitated with ab185955 at 1/50 dilution.

    Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

  • Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955)
    Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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