Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955)
![Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955)](https://www.abcam.com/ps/products/185/ab185955/Images/ab185955-219603-anti-sae2-uba2-antibody-epr14880-western-blot.jpg "Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14880] to SAE2 / UBA2
- Suitable for: IHC-P, IP, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-SAE2 / UBA2 antibody [EPR14880]
See all SAE2 / UBA2 primary antibodies -
Description
Rabbit monoclonal [EPR14880] to SAE2 / UBA2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P MouseHumanIP HumanWB MouseHuman
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- HeLa, A549, K562, Raw264.7 and PC-12 cell lysates; Human colon tissue; Mouse brain tissue; HeLa cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14880 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955)All lanes : Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A549 cell lysate
Lane 3 : K562 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 71 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
-
![Western blot - Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955)](https://www.abcam.com/ps/products/185/ab185955/Images/ab185955-219602-anti-sae2-uba2-antibody-epr14880-western-blot.jpg)
Western blot - Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955)All lanes : Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955) at 1/1000 dilution
Lane 1 : Raw264.7 cell lysate
Lane 2 : PC-12 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 71 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955)](https://www.abcam.com/ps/products/185/ab185955/Images/ab185955-219601-anti-sae2-uba2-antibody-epr14880-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955)Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SAE2 / UBA2 with ab185955 at 1/50 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955)](https://www.abcam.com/ps/products/185/ab185955/Images/ab185955-219600-anti-sae2-uba2-antibody-epr14880-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955)Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling SAE2 / UBA2 with ab185955 at 1/50 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
-
![Immunocytochemistry/ Immunofluorescence - Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955)](https://www.abcam.com/ps/products/185/ab185955/Images/ab185955-219599-anti-sae2-uba2-antibody-epr14880-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955)Immunofluorescent analysis of acetone-fixed HeLa cells labeling SAE2 / UBA2 with ab185955 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution.
-
![Immunoprecipitation - Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955)](https://www.abcam.com/ps/products/185/ab185955/Images/ab185955-219598-anti-sae2-uba2-antibody-epr14880-immunoprecipitation.jpg)
Immunoprecipitation - Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955)Western blot analysis of SAE2 / UBA2 in K562 cell lysate immunoprecipitated with ab185955 at 1/50 dilution.
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
-
![Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955)](https://www.abcam.com/ps/products/185/ab185955/Images/ab185955-7-benefits-of-recombinant-antibodies.png)
Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955)
