Anti-SAE2 / UBA2 antibody (ab241497)
Key features and details
- Rabbit polyclonal to SAE2 / UBA2
- Suitable for: IP, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-SAE2 / UBA2 antibody
See all SAE2 / UBA2 primary antibodies -
Description
Rabbit polyclonal to SAE2 / UBA2 -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human SAE2/ UBA2 aa 525-575. The exact sequence is proprietary. (NP_005490.1)
Database link: Q9UBT2 -
Positive control
- WB: HeLa, HEK-293T and NIH/3T3 cell lysate. IP: HeLa cell lysate. IHC-P: Human prostate carcinoma tissue; Mouse renal cell carcinoma tissue.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to SAE2 / UBA2 immobilized on solid support -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-SAE2 / UBA2 antibody (ab241497)All lanes : Anti-SAE2 / UBA2 antibody (ab241497) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 50 µg
Lane 2 : HeLa cell lysate at 15 µg
Lane 3 : HeLa cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate at 50 µg
Developed using the ECL technique.
Exposure time: 10 seconds
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAE2 / UBA2 antibody (ab241497)Formalin-fixed, paraffin-embedded human prostate carcinoma (left) and mouse renal cell carcinoma (right) tissues stained for SAE2 / UBA2 using ab241497 at 1/200 dilution in immunohistochemical analysis. DAB staining.
-
Immunoprecipitation - Anti-SAE2 / UBA2 antibody (ab241497)SAE2 / UBA2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241497 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab241497 at 1 µg/ml.
Lane 1: ab241497 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.Detection: Chemiluminescence with exposure time of 10 seconds.
