Anti-SAE2 / UBA2 antibody (ab95835)
Key features and details
- Rabbit polyclonal to SAE2 / UBA2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SAE2 / UBA2 antibody
See all SAE2 / UBA2 primary antibodies -
Description
Rabbit polyclonal to SAE2 / UBA2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human SAE2/ UBA2 aa 150-250 conjugated to keyhole limpet haemocyanin.
(Peptide available asab109093) -
Positive control
- This antibody gave a positive signal in both Human brain and Human heart tissue lysates aswell as the following whole cell lysates: HeLa; Jurkat; HepG2; HEK293; Caco2.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab95835 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatChickenCowDogZebrafishApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa).Target
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Function
The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. -
Pathway
Protein modification; protein sumoylation. -
Sequence similarities
Belongs to the ubiquitin-activating E1 family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 10054 Human
- Entrez Gene: 50995 Mouse
- Entrez Gene: 308508 Rat
- Entrez Gene: 406672 Zebrafish
- Omim: 613295 Human
- SwissProt: Q9UBT2 Human
- SwissProt: Q9Z1F9 Mouse
- SwissProt: Q7SXG4 Zebrafish
see all -
Alternative names
- Anthracycline associated resistance ARX antibody
- Anthracycline-associated resistance ARX antibody
- ARX antibody
see all
Images
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Western blot - Anti-SAE2 / UBA2 antibody (ab95835)All lanes : Anti-SAE2 / UBA2 antibody (ab95835) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 5 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 6 : Human brain tissue lysate - total protein (ab29466)
Lane 7 : Human heart tissue lysate - total protein (ab29431)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 71 kDa
Observed band size: 71 kDa
Exposure time: 20 minutes
Abcam recommends using milk as the blocking agent at a concentration of 3%. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
Datasheets and documents
References (0)
ab95835 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-SAE2 / UBA2 antibody (ab95835)All lanes : Anti-SAE2 / UBA2 antibody (ab95835) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 5 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 6 : Human brain tissue lysate - total protein (ab29466)
Lane 7 : Human heart tissue lysate - total protein (ab29431)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 71 kDa
Observed band size: 71 kDa
Exposure time: 20 minutes
Abcam recommends using milk as the blocking agent at a concentration of 3%. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
