Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SENP1 antibody [EPR3844] (ab108981)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3844] to SENP1
  • Suitable for: Flow Cyt, ICC/IF, WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-SENP1 antibody [EPR3844]
    See all SENP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR3844] to SENP1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HUVEC, Jurkat, Daudi and U87-MG cell lysates. IHC-P: Human testis tissue. ICC/IF: Jurkat cells. Flow Cyt: HeLa cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this SENP1 antibody (ab108981) may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-SENP1 antibody [EPR3844] (ab108981)
    Western blot - Anti-SENP1 antibody [EPR3844] (ab108981)
    Anti-SENP1 antibody [EPR3844] (ab108981) at 1/20000 dilution (purified) + Daudi cell lysate at 10 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

    Predicted band size: 73 kDa
    Observed band size: 73 kDa



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Immunocytochemistry/ Immunofluorescence - Anti-SENP1 antibody [EPR3844] (ab108981)
    Immunocytochemistry/ Immunofluorescence - Anti-SENP1 antibody [EPR3844] (ab108981)

    Immunofluorescence staining of Jurkat cells with purified ab108981 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab108981 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SENP1 antibody [EPR3844] (ab108981)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SENP1 antibody [EPR3844] (ab108981)
    Immunohistochemical staining of paraffin embedded human testis with purified ab108981 at a working dilution of 1/300. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Flow Cytometry - Anti-SENP1 antibody [EPR3844] (ab108981)
    Flow Cytometry - Anti-SENP1 antibody [EPR3844] (ab108981)

    Flow Cytometry analysis of HeLa cells labelling SENP1 with purified ab108981 at a dilution of 1/20 (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. An Alexa Flour® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

  • Immunocytochemistry/ Immunofluorescence - Anti-SENP1 antibody [EPR3844] (ab108981)
    Immunocytochemistry/ Immunofluorescence - Anti-SENP1 antibody [EPR3844] (ab108981) Image courtesy of an Abreview submitted by Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada

    Unpurified ab108981 (1/500) staining SENP1 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.

    See Abreview

  • Western blot - Anti-SENP1 antibody [EPR3844] (ab108981)
    Western blot - Anti-SENP1 antibody [EPR3844] (ab108981)
    Anti-SENP1 antibody [EPR3844] (ab108981) at 1/20000 dilution (purified) + U87-MG cell lysate at 10 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

    Predicted band size: 73 kDa
    Observed band size: 73 kDa



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-SENP1 antibody [EPR3844] (ab108981)
    Western blot - Anti-SENP1 antibody [EPR3844] (ab108981)
    All lanes : Anti-SENP1 antibody [EPR3844] (ab108981) at 1/1000 dilution (unpurified)

    Lane 1 : HeLa cell lysates
    Lane 2 : HUVEC cell lysates
    Lane 3 : Jurkat cell lysates
    Lane 4 : Daudi cell lysates
    Lane 5 : U87-MG cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 73 kDa

  • Western blot - Anti-SENP1 antibody [EPR3844] (ab108981)
    Western blot - Anti-SENP1 antibody [EPR3844] (ab108981) This image is courtesy of an Abreview submitted by Ragnhild Eskeland
    All lanes : Anti-SENP1 antibody [EPR3844] (ab108981) at 1/1000 dilution (unpurified)

    Lane 1 : COS-1 cell lysate
    Lane 2 : COS-1 cell lysate transfected with SENP1
    Lane 3 : COS-1 cell lysate transfected with SENP1 mutant

    Lysates/proteins at 20000 cells per lane.

    Secondary
    All lanes : HRP-conjugated donkey anti-rabbit IgG polyclonal at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 73 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?


    Exposure time: 6 minutes


    Blocked with 3% milk for 1 hour at 25°C.

    Incubated with the primary antibody for 18 hours at 4°C.

    See Abreview

  • Anti-SENP1 antibody [EPR3844] (ab108981)
    Anti-SENP1 antibody [EPR3844] (ab108981)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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