Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PRPF31 antibody [EPR14587] (ab188577)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14587] to PRPF31
  • Suitable for: WB, Flow Cyt, ICC/IF
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-PRPF31 antibody [EPR14587]
    See all PRPF31 primary antibodies

  • Description

    Rabbit monoclonal [EPR14587] to PRPF31

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa and Jurkat cell lysates; rat spleen lysate. HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PRPF31 antibody [EPR14587] (ab188577)
    Western blot - Anti-PRPF31 antibody [EPR14587] (ab188577)
    All lanes : Anti-PRPF31 antibody [EPR14587] (ab188577) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 55 kDa
    Observed band size: 55 kDa

  • Western blot - Anti-PRPF31 antibody [EPR14587] (ab188577)
    Western blot - Anti-PRPF31 antibody [EPR14587] (ab188577)
    Anti-PRPF31 antibody [EPR14587] (ab188577) at 1/1000 dilution + rat spleen at 10 µg

    Secondary
    goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 55 kDa
    Observed band size: 55 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PRPF31 antibody [EPR14587] (ab188577)
    Immunocytochemistry/ Immunofluorescence - Anti-PRPF31 antibody [EPR14587] (ab188577)

    Immunofluorescence analysis of, paraformaldehyde-fixed, HeLa cells labeling PRPF31 with ab188577 at a 1/250 dilution. As secondary antibody goat anti-rabbit IgG (Alexa Fluor®488) was used at a 1/200. In blue DAPI staining.

  • Flow Cytometry - Anti-PRPF31 antibody [EPR14587] (ab188577)
    Flow Cytometry - Anti-PRPF31 antibody [EPR14587] (ab188577)

    Flow cytometry analysis of paraformaldehyde-fixed HeLa cells labeling PRPF31 with ab188577 at a 1/240 dilution and secondary antibody goat anti-rabbit IgG (FITC, red) at a 1/150 dilution, or negative control rabbit IgG (green).

  • Anti-PRPF31 antibody [EPR14587] (ab188577)
    Anti-PRPF31 antibody [EPR14587] (ab188577)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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